Science Immunology

Supplementary Materials

Supplementary Material for:

Dendritic cell–targeted lentiviral vector immunization uses pseudotransduction and DNA-mediated STING and cGAS activation

Jocelyn T. Kim, Yarong Liu, Rajan P. Kulkarni, Kevin K. Lee, Bingbing Dai, Geoffrey Lovely, Yong Ouyang, Pin Wang, Lili Yang, David Baltimore*

*Corresponding authors. Email: baltimo{at}caltech.edu

Published 21 July 2017, Sci. Immunol. 2, eaal1329 (2017)
DOI: 10.1126/sciimmunol.aal1329

This PDF file includes:

  • Materials and Methods
  • Fig. S1. Flow cytometry gating strategies.
  • Fig. S2. LV-mediated GFP expression and activation of BMDCs are dosedependent.
  • Fig. S3. Mouse bone marrow–derived CD11c+CD11b+ cells are pseudotransduced and activated.
  • Fig. S4. Mouse cDCs are pseudotransduced in vivo.
  • Fig. S5. LV activation of DCs is independent of TLR4 and type I IFN signaling.
  • Fig. S6. Wild-type and mutant bone marrow–derived CD11c+CD11b+ cells are generated in GM-CSF culture.
  • Fig. S7. VSV-G viral fusion activates PI3K.
  • Fig. S8. Nonviral DNA in vector particle is primarily dsDNA, fragmented, and human genomic in origin.
  • Table S1. Antibodies used in this study.
  • Table S2. Primer sets used in this study.

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