Science Immunology

Supplementary Materials

Supplementary Material for:

Helicobacter species are potent drivers of colonic T cell responses in homeostasis and inflammation

Jiani N. Chai, Yangqing Peng, Sunaina Rengarajan, Benjamin D. Solomon, Teresa L. Ai, Zeli Shen, Justin S. A. Perry, Kathryn A. Knoop, Takeshi Tanoue, Seiko Narushima, Kenya Honda, Charles O. Elson, Rodney D. Newberry, Thaddeus S. Stappenbeck, Andrew L. Kau, Daniel A. Peterson, James G. Fox, Chyi-Song Hsieh*

*Corresponding authors. Email: chsieh{at}wustl.edu

Published 21 July 2017, Sci. Immunol. 2, eaal5068 (2017)
DOI: 10.1126/sciimmunol.aal5068

This PDF file includes:

  • Materials and Methods
  • Fig. S1. DSS + αIL10R treatment induces colitis and Teff cell responses.
  • Fig. S2. Gating strategy for TCR Tg transfer experiments in Figs. 1, 4A (CT2 and CT6), and 6 (B and C) (DP1).
  • Fig. S3. Effects of DSS + αIL10R–mediated colitis on the TCR repertoire.
  • Fig. S4. Gating strategy for T cell transfer experiments in Figs. 2D, 4A (CT9 and T7-1), and 6C (NT2 and CT7).
  • Fig. S5. CBir1 TCR Tg cells become Teff, and not Treg, cells during DSS + αIL10R colitis.
  • Fig. S6. Gating strategy for T cell hybridoma experiments in Figs. 3 (A, B, and D) and 6 (A and D) and figs. S7 (A and C), S8, and S11.
  • Fig. S7. Colonic Treg TCRs react to MA Helicobacter species.
  • Fig. S8. CT2/CT6/CT9/T7-1 TCRs do not react to other bacterial isolates tested.
  • Fig. S9. Gating strategy and confirmation of Helicobacter spp. inoculation in Fig. 4B.
  • Fig. S10. Quantification of 16S rRNA gene abundance in the lumen or MA preparation.
  • Fig. S11. Specificity of DP1/NT2/CT7 TCRs for Bacteroides spp.
  • Fig. S12. Gating strategy for Fig. 7A and fig. S13A.
  • Fig. S13. Bacteria-specific induction of colitis in lymphopenic mice.
  • Fig. S14. Helicobacter species are detected in fecal and colonoscopy samples from IBD patients.
  • Fig. S15. Isotype controls for antibodies used in this study.
  • Table S1. TRAV multiplex primer sequences.
  • Table S2. TRAV designation, CDR3 amino acid sequences, and in vitro bacterial reactivity of indicated TCR clones.
  • References (65, 66)

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Other Supplementary Material for this manuscript includes the following:

  • Table S3 Excel file containing source data for all the figure panels.

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