Science Immunology

Supplementary Materials

Supplementary Material for:

Zika virus activates de novo and cross-reactive memory B cell responses in dengue-experienced donors

Thomas F. Rogers, Eileen C. Goodwin, Bryan Briney, Devin Sok, Nathan Beutler, Alexander Strubel, Rebecca Nedellec, Khoa Le, Michael E. Brown, Dennis R. Burton,* Laura M. Walker*

*Corresponding authors. Email: laura.walker{at}adimab.com (L.M.W.); burton{at}scripps.edu (D.R.B.)

Published 18 August 2017, Sci. Immunol. 2, eaan6809 (2017)
DOI: 10.1126/sciimmunol.aan6809

This PDF file includes:

  • Fig. S1. Binding of acute serum to ZIKV and DENV1–4 viral particles and NS1 proteins.
  • Fig. S2. Plasmablast sorting.
  • Fig. S3. Binding of plasmablast-derived mAbs to ZIKV-Para by ELISA.
  • Fig. S4. Western blot analysis of mAb binding to ZIKV lysate.
  • Fig. S5. Heat map of mAb binding reactivity to recombinant ZIKV E, WNV E, DIII, and E-FL.
  • Fig. S6. mAb binding to recombinant ZIKV and DENV E proteins.
  • Fig. S7. ELISA binding of whole virus–specific clones to ZIKV and DENV.
  • Fig. S8. SHM of NS1-specific mAbs.
  • Fig. S9. Representative neutralization curves.
  • Fig. S10. Enhancement of ZIKV infection by plasmablast-derived mAbs isolated from DENV-experienced donors.
  • Fig. S11. Memory B cell sorting.
  • Fig. S12. Distribution of clonal lineage members across plasmablast and memory B cell subsets.
  • Fig. S13. Epitope mapping of ZIKV-specific mAbs derived from memory B cells.
  • Table S1. Clinical characteristics of ZIKV-infected donors.
  • Table S2. Binding properties of ZIKV E?specific mAbs isolated from plasmablasts.
  • Table S3. Binding characteristics of mAb 4G2.
  • Table S4. Sequences of ZV-67 competitor mAbs.
  • Table S5. Competition of ADI-24247 and ADI-24314 with each other and with control mAbs.
  • Table S6. Binding properties of NS1-specific mAbs isolated from plasmablasts.
  • Table S7. Neutralizing activity of selected plasmablast-derived mAbs.
  • Table S8. Binding properties of ZIKV E–specific mAbs isolated from memory B cells.
  • Table S9. Clonal lineages shared between plasmablast and memory B cell– derived antibodies.
  • Table S10. Neutralizing activity of selected memory B cell–derived mAbs.

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