Science Immunology

Supplementary Materials

Supplementary Material for:

Tfr cells lack IL-2Rα but express decoy IL-1R2 and IL-1Ra and suppress the IL-1–dependent activation of Tfh cells

Paul-Gydeon G. Ritvo, Guillame Churlaud, Valentin Quiniou, Laura Florez, Faustine Brimaud, Gwladys Fourcade, Encarnita Mariotti-Ferrandiz, David Klatzmann*

*Corresponding authors. Email: david.klatzmann{at}

Published 8 September 2017, Sci. Immunol. 2, eaan0368 (2017)
DOI: 10.1126/sciimmunol.aan0368

This PDF file includes:

  • Materials and Methods
  • Fig. S1. Representative flow cytometry gating for Tfol cells.
  • Fig. S2. IL-2 increases the number of splenic Treg cells.
  • Fig. S3. Expression of CD25 on Tfr and Tfh cells in three different genetic backgrounds.
  • Fig. S4. Tfh and Tfr cells have similar expressions of Bcl6 and CD25.
  • Fig. S5. Clustering of Treg, Tfr, and Tfh cells based on the expression of the entire 545-gene set from the NanoString mouse immunology panel.
  • Fig. S6. Cytokines, CD40L, and OX40 expression of Tfr, Treg, and Tfh cells.
  • Fig. S7. IL-1R2 and OX40 expression of Tfr, Treg, Teff, and Tfh cells.
  • Fig. S8. IL-1β production in coculture of B and Tfh cells.
  • Fig. S9. GCB representative gating after INS or OVA immunization.
  • Table S1. Exact P values of the asterisk symbols shown in figures.

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