Science Immunology

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Supplementary Material for:

Skin infections are eliminated by cooperation of the fibrinolytic and innate immune systems

William Santus, Simona Barresi, Francesca Mingozzi, Achille Broggi, Ivan Orlandi, Giulia Stamerra, Marina Vai, Alessandra M. Martorana, Alessandra Polissi, Julia R. Köhler, Ningning Liu, Ivan Zanoni,* Francesca Granucci*

*Corresponding authors. Email: francesca.granucci{at}unimib.it (F.G.); ivan.zanoni{at}childrens.harvard.edu (I.Z.)

Published 22 September 2017, Sci. Immunol. 2, eaan2725 (2017)
DOI: 10.1126/sciimmunol.aan2725

This PDF file includes:

  • Fig. S1. Expression of NFATc1, NFATc2, and NFATc3 in immune cells.
  • Fig. S2. Magnifications of the selected areas shown in Fig. 1C.
  • Fig. S3. Additional histological images of the abscess after C. albicans infection.
  • Fig. S4. Histology of the abscess 1 month after infection of NFATc2-deficient mice.
  • Fig. S5. Granulocyte and monocyte recruitment at the infection site of WT and NFATc2-deficient mice.
  • Fig. S6. Visualization of C. albicans at the infection site 6 to 8 days after infection.
  • Fig. S7. Additional histological images of α-SMA staining in skin sections of WT and NFATc2-deficient mice.
  • Fig. S8. Additional histological images of p-SMAD2,3 staining.
  • Fig. S9. Magnifications of the selected areas shown in Fig. 3D.
  • Fig. S10. Additional histological images of the abscesses after C. albicans infection in the presence of a TGF-β inhibitor.
  • Fig. S11. Additional histological images of IFN-γ staining.
  • Fig. S12. IFN-γ induces capsule digestion.
  • Fig. S13. Additional histological images of the abscesses after C. albicans infection in the presence of PAI-1.
  • Fig. S14. Inhibition of plasmin or MMP-3 interferes with C. albicans elimination.
  • Fig. S15. IFN-γ mRNA is up-regulated in the infected skin of RAG-2–deficient mice.
  • Fig. S16. DCs are necessary for the activation of NK cells in the presence of C. albicans in vitro.
  • Fig. S17. DC depletion after DT injection in DOG mice.
  • Fig. S18. Granulocyte and monocyte recruitment at the infection site of DOG mice treated or not with DT to deplete DCs.
  • Fig. S19. Additional histological images of IFN-γ staining in the infected skin of DOG mice.
  • Fig. S20. IL-12 production by BMDCs after C. albicans stimulation.
  • Fig. S21. Original Western blots shown in Fig. 3A.
  • Fig. S22. Original Western blots shown in Fig. 4 (E and F).
  • Fig. S23. Original Western blots shown in Fig. 5E.
  • Fig. S24. Original Western blots shown in Fig. 7C.
  • Fig. S25. Original Western blots shown in Fig. 7D.
  • Fig. S26. Representative isotype control stainings for immunohistochemical analyses.
  • Fig. S27. Gating strategies used in cytofluorimetric analyses.
  • Fig. S28. Representative isotype control stainings for cytofluorimetric analyses.

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Other Supplementary Material for this manuscript includes the following:

  • Raw data for Figs. 1 (B, D, and E), 4 (A, C, and G), 5 (A, C, F, G, H, and J), 6 (B to I), and 7A; and figs. S1 (A and B), S5, S14, S15, S16, S17A, S18, and S20 (Microsoft Excel format).

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