Science Immunology

Supplementary Materials

Supplementary Material for:

CD169+ macrophages orchestrate innate immune responses by regulating bacterial localization in the spleen

Oriana A. Perez, Stephen T. Yeung, Paola Vera-Licona, Pablo A. Romagnoli, Tasleem Samji, Basak B. Ural, Leigh Maher, Masato Tanaka, Kamal M. Khanna*

*Corresponding author. Email: kamal.khanna{at}nyumc.org

Published 6 October 2017, Sci. Immunol. 2, eaah5520 (2017)
DOI: 10.1126/sciimmunol.aah5520

This PDF file includes:

  • Materials and Methods
  • Fig. S1. Characterization of WT and CD169-DTR mice.
  • Fig. S2. Splenic CD169+ macrophages are essential for protection against various blood-borne pathogens.
  • Fig. S3. Sorting strategy and quality control for RNA sequencing.
  • Fig. S4. CD169-DTR mice have elevated but displaced cytokine production.
  • Fig. S5. CD169+ macrophages recruit CD8α+ DCs to early sites of infection.
  • Fig. S6. Characterization of CD8α+ DCs in CD169-DTR mice.
  • Fig. S7. Myeloid cell differentials comparing Batf3−/− knockout and CD169- DTR–Batf3−/− knockout mice.
  • Fig. S8. Model depicting the role of CD169+ macrophages after Lm infection.
  • Table S1. Top 15 GO enriched biological processes up-regulated in CD169+ macrophages versus red pulp macrophages at steady state.
  • Table S2. Top 15 GO enriched biological processes up-regulated in CD169+ macrophages during infection versus steady state.
  • Table S3. Top 15 GO enriched biological processes down-regulated in CD169+ macrophages during infection versus steady state.
  • Table S4. List of identified TF candidates for up-regulated genes in CD169+ macrophages isolated from infected mice versus uninfected mice.
  • Table S5. Enriched immune-related GO terms for corresponding TFs in CD169+ macrophages during infection versus steady state.
  • Table S6. Antibodies used for fluorescence-activated cell sorting (FACS) and confocal microscopy.
  • Legends for movies S1 to S3
  • References (36–38)

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Other Supplementary Material for this manuscript includes the following:

  • Movie S1 (.mov format). Listeria is captured by CD169+ macrophages early after infection.
  • Movie S2 (.mov format). Listeria is captured by CD169+ macrophages early after infection.
  • Movie S3 (.mov format). CD169+ macrophages transfer bacteria to CD8α+ DCs. Source data (Excel file)
  • Source data (Excel file).

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