Science Immunology

Supplementary Materials

Supplementary Material for:

Enzymatic synthesis of core 2 O-glycans governs the tissue-trafficking potential of memory CD8+ T cells

Jossef F. Osborn, Jana L. Mooster, Samuel J. Hobbs, Michael W. Munks, Conrad Barry, John T. Harty, Ann B. Hill, Jeffrey C. Nolz*

*Corresponding author. Email: nolz{at}

Published 13 October 2017, Sci. Immunol. 2, eaan6049 (2017)
DOI: 10.1126/sciimmunol.aan6049

This PDF file includes:

  • Materials and Methods
  • Fig. S1. Trafficking of memory CD8+ T cells into VacV-infected skin.
  • Fig. S2. CD8+ T cells require Gcnt1 to synthesize core 2 O-glycans for binding to E- and P-selectins.
  • Fig. S3. Trafficking of memory CD8+ T cells into VacV-infected skin requires interactions with E- and P-selectins.
  • Fig. S4. IL-15 regulates E- and P-selectin binding during VacV infection.
  • Fig. S5. TCM CD8+ T cells synthesize core 2 O-glycans and maintain expression of CD62L.
  • Fig. S6. Phenotype of terminally differentiated tertiary memory CD8+ T cells using repetitive LCMV-Armstrong infection.
  • Fig. S7. Primary memory CD8+ T cells are recruited into the skin better than tertiary memory CD8+ T cells during contact hypersensitivity.
  • Table S1. Primer pairs for gene expression analysis in Fig. 2 and fig. S4.

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Other Supplementary Material for this manuscript includes the following:

  • Table S2 (Microsoft Excel format). Raw data sets and statistical analyses..

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