Science Immunology

Supplementary Materials

Supplementary Material for:

Interleukin-10 from CD4+ follicular regulatory T cells promotes the germinal center response

Brian J. Laidlaw, Yisi Lu, Robert A. Amezquita, Jason S. Weinstein, Jason A. Vander Heiden, Namita T. Gupta, Steven H. Kleinstein, Susan M. Kaech, Joe Craft*

*Corresponding author. Email: joseph.craft{at}yale.edu

Published 20 October 2017, Sci. Immunol. 2, eaan4767 (2017)
DOI: 10.1126/sciimmunol.aan4767

This PDF file includes:

  • Supplementary Methods
  • Fig. S1. Flow cytometric gating strategy for Tfh, pre-Tfh, GC B, Treg, and Tfr cells, with IL-10 expression by these populations.
  • Fig. S2. Mice lacking regulatory CD4+ T cell–derived IL-10 do not have defects in steady-state lymphoid cell populations.
  • Fig. S3. Temporal development of the GC B cell response in mice lacking regulatory CD4+ T cell–derived IL-10.
  • Fig. S4. Systemic IL-10 is not required for the GC response.
  • Fig. S5. Regulatory CD4+ T cell–derived IL-10 is not required for effector CD4+ T cell differentiation.
  • Fig. S6. Heat map of DEGs based on RNA-seq.
  • Fig. S7. GC B cells in mice lacking regulatory CD4+ T cell–derived IL-10 display similar levels of proliferation and death.
  • Fig. S8. IL-10 does not induce FOXO1 nuclear translocation in IgDhi B cells.
  • Fig. S9. The VH CDR3 region of GC B cells in mice lacking regulatory CD4+ T cell–derived IL-10 displays altered amino acid physiochemical properties.
  • References (57–68)

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Other Supplementary Material for this manuscript includes the following:

  • Table S1 (Microsoft Excel format). Tabulated data for Figs. 1 to 6 and figs. S1 to S9.

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