Science Immunology
Supplementary Materials
A molecular basis of human T cell receptor autoreactivity toward self-phospholipids
Adam Shahine, Ildiko Van Rhijn, Tan-Yun Cheng, Sarah Iwany, Stephanie Gras,* D. Branch Moody,* Jamie Rossjohn*
*Corresponding authors. Email: stephanie.gras{at}monash.edu (S.G.); bmoody{at}partners.org (D.B.M.); jamie.rossjohn{at}monash.edu (J.R.)
Published 20 October 2017, Sci. Immunol. 2, eaao1384 (2017)
DOI: 10.1126/sciimmunol.aao1384
This PDF file includes:
- Fig. S1. TCR tetramer validation.
- Fig. S2. Representative gating strategy for Fig. 1B.
- Fig. S3. Major lipid classes produced by C1R and K562 cells.
- Fig. S4. TLC-MS analysis of C1R lipids nearly comigrating with a PG standard.
- Fig. S5. Quantification of PG in cells or eluted from CD1b.
- Fig. S6. Nanoelectrospray analysis of protein eluates.
- Fig. S7. Loading of phospholipids in CD1b molecule.
- Fig. S8. Electron density maps for the phospholipid antigens presented by CD1b.
- Fig. S9. Phospholipid anchoring residues within CD1b pockets.
- Fig. S10. Sequence alignments of TCR Vα and Vβ regions.
- Table S1. Data collection and refinement statistics.
- Table S2. Contacts between PG90 TCR and CD1b-PG.
Other Supplementary Material for this manuscript includes the following:
- Table S3 (Microsoft Excel format). Raw data for Fig. 3A.
Files in this Data Supplement: