Supplementary Material for:

A molecular basis of human T cell receptor autoreactivity toward self-phospholipids

Adam Shahine, Ildiko Van Rhijn, Tan-Yun Cheng, Sarah Iwany, Stephanie Gras,* D. Branch Moody,* Jamie Rossjohn*

*Corresponding authors. Email: stephanie.gras{at}monash.edu (S.G.); bmoody{at}partners.org (D.B.M.); jamie.rossjohn{at}monash.edu (J.R.)

Published 20 October 2017, Sci. Immunol. 2, eaao1384 (2017)
DOI: 10.1126/sciimmunol.aao1384

This PDF file includes:

• Fig. S1. TCR tetramer validation.
• Fig. S2. Representative gating strategy for Fig. 1B.
• Fig. S3. Major lipid classes produced by C1R and K562 cells.
• Fig. S4. TLC-MS analysis of C1R lipids nearly comigrating with a PG standard.
• Fig. S5. Quantification of PG in cells or eluted from CD1b.
• Fig. S6. Nanoelectrospray analysis of protein eluates.
• Fig. S7. Loading of phospholipids in CD1b molecule.
• Fig. S8. Electron density maps for the phospholipid antigens presented by CD1b.
• Fig. S9. Phospholipid anchoring residues within CD1b pockets.
• Fig. S10. Sequence alignments of TCR Vα and Vβ regions.
• Table S1. Data collection and refinement statistics.
• Table S2. Contacts between PG90 TCR and CD1b-PG.

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Other Supplementary Material for this manuscript includes the following:

• Table S3 (Microsoft Excel format). Raw data for Fig. 3A.