Science Immunology

Supplementary Materials

Supplementary Material for:

Lysosome signaling controls the migration of dendritic cells

Marine Bretou,* Pablo J. Sáez, Doriane Sanséau, Mathieu Maurin, Danielle Lankar, Melanie Chabaud, Carmine Spampanato, Odile Malbec, Lucie Barbier, Shmuel Muallem, Paolo Maiuri, Andrea Ballabio, Julie Helft, Matthieu Piel, Pablo Vargas,* Ana-Maria Lennon-Duménil*

*Corresponding author. Email: marine.bretou{at}curie.fr (M.B.); pablo.vargas{at}curie.fr (P.V.); amlennon{at}curie.fr (A.-M.L.-D.)

Published 27 October 2017, Sci. Immunol. 2, eaak9573 (2017)
DOI: 10.1126/sciimmunol.aak9573

This PDF file includes:

  • Materials and Methods
  • Fig. S1. TFEB translocates to the nucleus and is required for fast migration of LPS-DCs (related to Fig. 1).
  • Fig. S2. TRPML1 is required for fast DC migration, chemotaxis, and arrival at LNs (related to Fig. 2).
  • Fig. S3. Fast DC migration relies on calcium release by TRPML1 (related to Fig. 3).
  • Fig. S4. TFEB controls actin cytoskeleton reorganization in response to microbial sensing (related to Fig. 4).
  • Fig. S5. TRPML1 controls the organization of the DC actin cytoskeleton (related to Fig. 5).
  • Fig. S6. Calcium released through TRPML1 controls myosin IIA retrograde flow at the rear of LPS-DCs (related to Fig. 6).
  • Fig. S7. Inhibition of macropinocytosis activates the TRPML1-TFEB axis (related to Fig. 7).
  • Fig. S8. Fascin expression, lysosomal area, and cholesterol homeostasis in migrating DCs.
  • Legend for table S1
  • Legends for movies S1 to S6
  • References (44–52)

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Other Supplementary Material for this manuscript includes the following:

  • Table S1 (Microsoft Excel format). Source data for all graphs with n < 25.
  • Movie S1 (.mp4 format). F-actin is in proximity to the endolysosomes at the rear of the LPS-DCs.
  • Movie S2 (.mp4 format). F-actin dynamics in control (siCtrl) and TFEB-silenced (siTFEB) LPS-DCs.
  • Movie S3 (.mp4 format). F-actin dynamics in Trpml1+/+ and Trpml1−/− LPS-DCs.
  • Movie S4 (.mp4 format). Myosin IIA?GFP dynamics in Trpml1+/+ and Trpml1−/− LPS-DCs.
  • Movie S5 (.mp4 format). F-actin dynamics in LPS-DCs treated with DMSO or para-nitroblebbistatin.
  • Movie S6 (.mp4 format). Myosin IIA moves along actin cables.

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