Science Immunology

Supplementary Materials

Supplementary Material for:

A melanin-mediated cancer immunotherapy patch

Yanqi Ye, Chao Wang, Xudong Zhang, Quanyin Hu, Yuqi Zhang, Qi Liu, Di Wen, Joshua Milligan, Adriano Bellotti, Leaf Huang, Gianpietro Dotti, Zhen Gu*

*Corresponding author. Email: zgu{at}email.unc.edu

Published 10 November 2017, Sci. Immunol. 2, eaan5692 (2017)
DOI: 10.1126/sciimmunol.aan5692

This PDF file includes:

  • Materials and Methods
  • Fig. S1. Mechanical property of the MN.
  • Fig. S2. Characteristics of tumor lysate solution and synthetic melanin.
  • Fig. S3. Heating behavior of MN patches by repetitive NIR irradiation.
  • Fig. S4. Surface temperature of MN patches with various loadings of tumor lysates upon NIR irradiation.
  • Fig. S5. In vitro release profiles of GM-CSF and tumor lysate proteins.
  • Fig. S6. Scanning electron microscopy images of MN patch after insertion into the mouse skin over time.
  • Fig. S7. DC function evaluation after in vitro activation.
  • Fig. S8. Cytotoxicity study of the blank MNs.
  • Fig. S9. Characterization of the skin after MN insertion.
  • Fig. S10. Melanin-loaded MNs confer protective immunity in vivo.
  • Fig. S11. Tumor growth in control and treated mice.
  • Fig. S12. Quantified B16F10 bioluminescent tumor signals in control and treated mice.
  • Fig. S13. Tumor weights in control and treated mice.
  • Fig. S14. Histology and apoptosis analysis of the tumor sections.
  • Fig. S15. Tumor growth of mice receiving the transdermal cancer immunotherapy.
  • Fig. S16. Measurement of local microcirculatory blood perfusion of mice.
  • Fig. S17. Immunologic responses after the transdermal cancer immunotherapy.
  • Fig. S18. Quantification of IgG1 subtypes in serum after treatment with blank, MN, or MN + NIR.
  • Fig. S19. ROS detection by flow cytometry in tumor sections.
  • Fig. S20. HSP90 expression after the transdermal cancer immunotherapy.
  • Fig. S21. Cytokine kinetics after the transdermal cancer immunotherapy.
  • Fig. S22. Histology analysis after the transdermal cancer immunotherapy.
  • Fig. S23. Antitumor effect of the transdermal cancer immunotherapy toward different tumor models.
  • Fig. S24. Surface temperature changes of the melanin-loaded MNs.
  • Fig. S25. Antitumor effect of the transdermal cancer immunotherapy.
  • Fig. S26. HSP70 expression after the transdermal cancer immunotherapy.
  • Fig. S27. Representative quantitative analysis of the DC activation.
  • Fig. S28. Cytokine kinetics after the transdermal cancer immunotherapy.
  • Fig. S29. Average weights of mice after the transdermal cancer immunotherapy in control and treated mice.
  • Fig. S30. H&E staining of organs collected after the transdermal cancer immunotherapy.
  • Table S1. Melanin content of tumors excised from tumor-bearing mice.
  • Table S2. Measurement of total local microcirculatory blood perfusion of mice receiving different treatments using the laser Doppler flowmetry.

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