Science Immunology

Supplementary Materials

Supplementary Material for:

Suppression of FIP200 and autophagy by tumor-derived lactate promotes na?ve T cell apoptosis and affects tumor immunity

Houjun Xia, Wei Wang, Joel Crespo, Ilona Kryczek, Wei Li, Shuang Wei, Zhaoqun Bian, Tomasz Maj, Mingxiao He, Rebecca J. Liu, Youwen He, Ramandeep Rattan, Adnan Munkarah, Jun-Lin Guan, Weiping Zou*

*Corresponding author. Email: wzou{at}med.umich.edu

Published 17 November 2017, Sci. Immunol. 2, eaan4631 (2017)
DOI: 10.1126/sciimmunol.aan4631

This PDF file includes:

  • Materials and Methods
  • Fig. S1. FIP200 loss links to poor autophagy and high apoptosis in naïve T cells in tumor.
  • Fig. S2. Genetic FIP200 deletion impairs autophagy induction and causes T cell apoptosis.
  • Fig. S3. FIP200 deficiency alters mitochondria activation and ROS production in T cells.
  • Fig. S4. Impaired antitumor immunity in Fip200−/− mice.
  • Fig. S5. FIP200 controls Bak expression via maintaining microRNA1198-5p expression.
  • Fig. S6. FIP200 maintains microRNA1198-5p expression via Ago2 in naïve T cells.
  • Fig. S7. Tumor-derived lactate suppresses FIP200 expression in naïve T cells. Legend for table S1
  • Table S2. Real-time PCR primers.

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Other Supplementary Material for this manuscript includes the following:

  • Table S1 (Microsoft Excel format). MicroRNA arrays in Fip200−/− and WT naïve CD4+ T cells.
  • Excel 1 Raw data.

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