Science Immunology

Supplemental Materials

Supplementary Material for:

GPR55 regulates intraepithelial lymphocyte migration dynamics and susceptibility to intestinal damage

Hayakazu Sumida, Erick Lu, Hsin Chen, Qiyun Yang, Ken Mackie, Jason G. Cyster*

*Corresponding author. Email: jason.cyster{at}ucsf.edu

Published 8 December 2017, Sci. Immunol. 2, eaao1135 (2017)
DOI: 10.1126/sciimmunol.aao1135

This PDF file includes:

  • Fig. S1. T cell populations and phenotype of GPR55-deficient and control gut or lymphoid tissues.
  • Fig. S2. GPR55 mediates migration inhibition.
  • Fig. S3. Detection of LPI species in the small intestine and spleen by LC-MS/MS.
  • Fig. S4. Movement of GPR55 KO T cells in close association with epithelial cells.
  • Fig. S5. Quantitation of PGE2 in the small intestine.
  • Fig. S6. Distribution of γδT IELs in indomethacin-treated mouse small intestine.
  • Fig. S7. Frequencies of conventional T cells in PPs, mLN, and blood after indomethacin treatment, and induction of gut homing receptors on cultured CD8 T cells.
  • Legends for movies S1 to S8

Download PDF

Other Supplementary Material for this manuscript includes the following:

  • Movie S1 (.mov format). Real-time imaging of CD8α+ IEL migration in Gpr55−/− small intestine.
  • Movie S2 (.mov format). Real-time imaging of CD8α+ IEL migration in Gpr55−/− small intestine.
  • Movie S3 (.mov format). Real-time imaging of TCRδ-eGFP IEL migration in Gpr55+/− small intestine.
  • Movie S4 (.mov format). Real-time imaging of TCRδ-eGFP IEL migration in Gpr55−/− small intestine.
  • Movie S5 (.mov format). Real-time imaging of TCRδ-eGFP IEL migration in Gpr55+/− small intestine (single z-plane movie).
  • Movie S6 (.mov format). Real-time imaging of TCRδ-eGFP IEL migration in Gpr55−/− small intestine (single z-plane movie).
  • Movie S7 (.mov format). Real-time imaging of TCRδ-eGFP IEL migration in the small intestine of a Rag1−/− mouse reconstituted with a mixture of Gpr55+/− Tcrβ−/− and Gpr55−/− Tcrβ−/− TCRδ-eGFP BM.
  • Movie S8 (.mov format). Real-time imaging of TCRδ-eGFP IEL migration in the small intestine of a Rag1−/− mouse reconstituted with a mixture of Gpr55+/− Tcrβ−/− and Gpr55−/− Tcrβ−/− TCRδ-eGFP BM (single z-plane movie).
  • Source data (Microsoft Excel format)

Files in this Data Supplement: