Science Immunology

Supplementary Materials

Supplementary Material for:

Production of BMP4 by endothelial cells is crucial for endogenous thymic regeneration

Tobias Wertheimer, Enrico Velardi, Jennifer Tsai, Kirsten Cooper, Shiyun Xiao, Christopher C. Kloss, Katja J. Ottmüller, Zeinab Mokhtari, Christian Brede, Paul deRoos, Sin?ad Kinsella, Brisa Palikuqi, Michael Ginsberg, Lauren F. Young, Fabiana Kreines, Sophia R. Lieberman, Amina Lazrak, Peipei Guo, Florent Malard, Odette M. Smith, Yusuke Shono, Robert R. Jenq, Alan M. Hanash, Daniel J. Nolan, Jason M. Butler, Andreas Beilhack, Nancy R. Manley, Shahin Rafii, Jarrod A. Dudakov,* Marcel R. M. van den Brink*

*Corresponding author. Email: jdudakov{at}fredhutch.org; vandenbm{at}mskcc.org

Published 12 January 2018, Sci. Immunol. 2, eaal2736 (2017)
DOI: 10.1126/sciimmunol.aal2736

This PDF file includes:

  • Fig. S1. Thymic response to damage.
  • Fig. S2. Distribution of BMPR subunits on thymic cell populations.
  • Fig. S3. Inhibition of BMP signaling abrogates endogenous thymic regeneration.
  • Fig. S4. Deletion of BMP4 in ECs abrogates endogenous thymic regeneration.
  • Fig. S5. Damage response in the thymus to corticosteroids, chemotherapy, and TBI.
  • Fig. S6. exECs can be propagated ex vivo and maintain an EC phenotype.
  • Fig. S7. Validating methods of inducing Foxn1 and silencing Bmp4.
  • Table S1. High-confidence FOXN1 target gene changes after TBI.

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Other Supplementary Material for this manuscript includes the following:

  • Movie S1. (.mp4 format). Thymic vascular architecture and branching by LSFM (day 0).
  • Movie S2. (.mp4 format). Thymic vascular architecture and branching by LSFM (day 4).
  • Movie S3. (.mp4 format). Thymic vascular architecture and branching by LSFM (day 7).
  • Movie S4. (.mp4 format). Thymic vascular architecture and branching by LSFM (day 14).

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