Science Immunology

Supplementary Materials

Supplementary Material for:

TLR7 escapes X chromosome inactivation in immune cells

Mélanie Souyris, Claire Cenac, Pascal Azar, Daniéle Daviaud, Astrid Canivet, Solange Grunenwald, Catherine Pienkowski, Julie Chaumeil, José E. Mejía, Jean-Charles Guéry*

*Corresponding author. Email: jean-charles.guery{at}inserm.fr

Published 26 January 2018, Sci. Immunol. 3, eaap8855 (2017)
DOI: 10.1126/sciimmunol.aap8855

This PDF file includes:

  • Materials and Methods
  • Fig. S1. Determination of TLR7 monoallelic or biallelic expression at the singlecell level.
  • Fig. S2. In situ visualization of monoallelic and biallelic TLR7 transcripts at the single-cell level.
  • Fig. S3. Relationship between transcript quantitation and TLR7 monoallelic or biallelic expression.
  • Fig. S4. Specific Western blot immunodetection of TLR7.
  • Fig. S5. Unstable TLR7 allelic expression in EBV-transformed B cells.
  • Table S1. PCR primer pairs used in the allele-of-origin assay.
  • Table S2. Primary antibodies.
  • Table S3. PCR primer pairs used in the preparation of the TLR7 RNA FISH probe.
  • Table S4. PCR primer pairs used in the preparation of the XIST RNA FISH probe.
  • Table S5. PCR primer pairs used in the preparation of the SLC25A6 RNA FISH probe.
  • Table S6. 2 ? 2 contingency tables of TLR7 biallelic and monoallelic cell counts in IgG+ and IgG B cells.
  • Legend for movie S1
  • References (52—54)

Download PDF

Other Supplementary Material for this manuscript includes the following:

  • Movie S1 (.avi format). RNA FISH visualization of TLR7 escape from X inactivation in a memory B lymphocyte.

Files in this Data Supplement: