Science Immunology

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Supplementary Material for:

Islet-reactive CD8+ T cell frequencies in the pancreas, but not in blood, distinguish type 1 diabetic patients from healthy donors

Slobodan Culina, Ana Ines Lalanne, Georgia Afonso, Karen Cerosaletti, Sheena Pinto, Guido Sebastiani, Klaudia Kuranda, Laura Nigi, Anne Eugster, Thomas Østerbye, Alicia Maugein, James E. McLaren, Kristin Ladell, Etienne Larger, Jean-Paul Beressi, Anna Lissina, Victor Appay, Howard W. Davidson, Søren Buus, David A. Price, Matthias Kuhn, Ezio Bonifacio, Manuela Battaglia, Sophie Caillat-Zucman, Francesco Dotta, Raphael Scharfmann, Bruno Kyewski, Roberto Mallone,* ImMaDiab Study Group

*Corresponding author. Email: roberto.mallone{at}inserm.fr

Published 2 February 2018, Sci. Immunol. 3, eaao4013 (2017)
DOI: 10.1126/sciimmunol.aao4013

This PDF file includes:

  • Fig. S1. Cytokine secretion and cytotoxicity of ZnT8186?194-reactive CD8+ T cells from T1D patient D222D.
  • Fig. S2. CD8+ T cell recognition and HLA-A2 binding of ZnT8186?194 and ZnT8185?194 epitope variants.
  • Fig. S3. Ag sensitivity correlates with Ag avidity and polyfunctionality in ZnT8186?194-reactive CD8+ T cell clones.
  • Fig. S4. Modulation of HLA class I and ZnT8 expression in human β cell lines.
  • Fig. S5. TCR sequences of ZnT8186?194-reactive CD8+ T cell clones.
  • Fig. S6. ZnT8186?194-reactive clonotype-specific TaqMan assays.
  • Fig. S7. Gating strategy for the analysis of ZnT8186?194, MelanA26?35, and Flu MP58?66 MMr+CD8+ T cells.
  • Fig. S8. IFN-γ secretion by ZnT8186?194-reactive CD8+ T cells.
  • Fig. S9. Gene expression in ex vivo single-sorted ZnT8186?194 MMr+CD8+ T cells.
  • Fig. S10. Extended combinatorial MMr panel for the analysis of multiple isletreactive CD8+ T cell populations, and reproducibility of ex vivo MMr assays.
  • Fig. S11. CD27, CD28, and CD95 expression on ZnT8186?194-reactive CD8+ T cells.
  • Fig. S12. Representative MMr and CD45RA/CCR7 dot plots for HLA-A2+ and HLA-A2 healthy donors depicted in Fig. 6 (F and G).
  • Fig. S13. Correlation between the frequency of MMr+CD8+ T cells and the Agexperienced fraction within the same MMr+CD8+ population.
  • Table S1. Summary of ZnT8186?194-reactive CD8+ T cell clones.
  • Table S2. Characteristics of study patients for in silico TRB analyses.
  • Table S3. Characteristics of HLA-A2+ study patients for ex vivo MMr studies.
  • Table S4. Characteristics of HLA-A2+ and HLA-A2 healthy donors for ex vivo MMr studies.
  • Table S5. Characteristics of nPOD cases for in situ ZnT8186?194 MMr staining.
  • Table S6. Primers used for gene expression analysis of the individual ZnT8186?194 MMr+CD8+ T cells depicted in fig. S9A.
  • Members of the ImMaDiab Study Group
  • Reference (43)

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