Science Immunology

Supplementary Materials

Supplementary Material for:

Clonal selection drives protective memory B cell responses in controlled human malaria infection

Rajagopal Murugan, Lisa Buchauer, Gianna Triller, Cornelia Kreschel, Giulia Costa, Gemma Pidelaserra Martí, Katharina Imkeller, Christian E. Busse, Sumana Chakravarty, B. Kim Lee Sim, Stephen L. Hoffman, Elena A. Levashina, Peter G. Kremsner, Benjamin Mordm?ller, Thomas Höfer,* Hedda Wardemann*

*Corresponding author. Email: t.hoefer{at}dkfz-heidelberg.de (T.H.); h.wardemann{at}dkfz.de (H.W.)

Published 16 February 2018, Sci. Immunol. 3, eaap8029 (2017)
DOI: 10.1126/sciimmunol.aap8029

This PDF file includes:

  • Fig. S1. Anti-PfCSP response.
  • Fig. S2. PfCSP memory B cell Ig gene sequence analysis and antibody function.
  • Fig. S3. Inefficient affinity maturation over repeated Pf exposure.
  • Fig. S4. Influence of GC seeder cell frequencies and antigen complexity (nkey) on clonal evolution within individual GCs.
  • Fig. S5. Avalanche effect over three successive infections in an exemplary system of 10 different GC sites.
  • Fig. S6. Repertoire and Ig gene feature analysis of antibodies from PfCSP memory B cells and plasmablasts.
  • Table S1. Number of sequenced and cloned PfCSP-reactive memory B cell antibodies.
  • Table S2. Number of sequenced plasmablast antibodies.
  • Table S3. Clonally expanded PfCSP-reactive memory B cell clusters.
  • Table S4. Simulation parameters, binding model, GC simulation dynamics.
  • Table S5. PfCSP-reactive memory B cell antibodies with 8–amino acid–long KCDR3.

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