Science Immunology

Supplementary Materials

Supplementary Material for:

RORα-expressing T regulatory cells restrain allergic skin inflammation

Nidhi Malhotra,* Juan Manuel Leyva-Castillo,* Unmesh Jadhav, Olga Barreiro, Christy Kam, Nicholas K. O′Neill, Francoise Meylan, Pierre Chambon, Ulrich H. von Andrian, Richard M. Siegel, Eddie C. Wang, Ramesh Shivdasani, Raif S. Geha*

*Corresponding author. Email: nidhi.malhotra{at}elstartherapeutics.com (N.M.); manuel.leyvacastillo{at}childrens.harvard.edu (J.M.L.-C.); raif.geha{at}childrens.harvard.edu (R.S.G.)

Published 2 March 2018, Sci. Immunol. 3, eaao6923 (2018)
DOI: 10.1126/sciimmunol.aao6923

This PDF file includes:

  • Fig. S1. RORα-expressing skin Tregs are HELIOS+ natural Tregs that express high levels of ST2 and ICOS.
  • Fig. S2. Multiple skin-resident cell types express RORα.
  • Fig. S3. CD4+ T cells are the only cells that express eGFP in Foxp3egfp mice.
  • Fig. S4. Treg-specific RORα deficiency does not affect Treg numbers in the skin, nor their ability to produce IL-10.
  • Fig. S5. Skin TSLP and serum IgE levels in Foxp3eyfp-creRorafl/fl mice and Foxp3eyfp-cre controls.
  • Fig. S6. Effect of lack of RORα in Tregs on MC903-driven blood eosinophilia and on IL-4, IL-13, and chemokines in MC903-treated skin.
  • Fig. S7. Analysis of correlation of RNA-seq samples.
  • Fig. S8. Increased number and motility of Tregs in MC903-treated ear skin.
  • Table S1. Summary of RNA-seq experiments.
  • Legends for movies S1 and S2

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Other Supplementary Material for this manuscript includes the following:

  • Movie S1 (.mov format). Intravital two-photon imaging of the untreated ear dermis of a Foxp3egfp animal.
  • Movie S2 (.mov format). Intravital two-photon imaging of the MC903-treated ear dermis of Foxp3egfp mice.
  • Data file (Microsoft Excel format)

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