Science Immunology

The receptor repertoire and functional profile of follicular T cells in HIV-infected lymph nodes

Ben S. Wendel, Daniel del Alcazar, Chenfeng He, Perla M. Del Río-Estrada, Benjamas Aiamkitsumrit, Yuria Ablanedo-Terrazas, Stefany M. Hernandez, Ke-Yue Ma, Michael R. Betts, Laura Pulido, Jun Huang, Phyllis A. Gimotty, Gustavo Reyes-Terán, Ning Jiang,* Laura F. Su*

*Corresponding author. Email: laurasu{at}upenn.edu (L.F.S.); jiang{at}austin.utexas.edu (N.J.)

Published 6 April 2018, Sci. Immunol. 3, eaan8884 (2018)
DOI: 10.1126/sciimmunol.aan8884

This PDF file includes:

  • Methods
  • Fig. S1. GC TFH cells are clonally expanded.
  • Fig. S2. Antigen-driven clonal selection signature in GC TFH cells of HIVinfectedLNs.
  • Fig. S3. Identification of Gag- or HA-reactive T cells in cultured cells.
  • Fig. S4. HA-specific CD4 T cell clones detected in HIV-infected LNs.
  • Fig. S5. Batch normalization of CyTOF data using calibration beads.
  • Fig. S6. Identification of GC TFH cells from LN samples.
  • Fig. S7. High-dimensional analysis of CXCR5+CD45RO+CD4+ T cells.
  • Fig. S8. Signal intensity of individual markers on t-SNE plots.
  • Fig. S9. Correlation between IL-21 frequency and CD38 expression with viral load and antiviral treatment.
  • Fig. S10. IL-21 surface capture effectively identified IL-21–producing T cells.
  • Fig. S11. Correlation between the frequency of IL-21+ GC TFH cells and signal intensity of CD57 staining.
  • Fig. S12. TFH cells respond to Gag or HA peptides.
  • Fig. S13. Identification of effector molecule–producing CD4+ T cells on CyTOF.
  • Fig. S14. Identification of effector molecule–producing CD4+ T cells by flow cytometry.
  • Fig. S15. Frequency of IL-21–producing subsets in TFH cells in HIV+ and HC samples.
  • Fig. S16. Identification of B cell subsets.
  • Table S1. TCRβ sequencing primers.
  • Table S2. TCR repertoire sequencing cell and transcript counts.
  • Table S3. Gag and HA TCR sequence reference panel.
  • Table S4. CyTOF antibody staining panel.
  • Table S5. Clinical characteristics and demographic information of LN samples.
  • Table S6. Gag-reactive TCR sequences from single-cell TCR sequencing of IL-21+ cells.

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