Science Immunology

Supplementary Materials

Supplementary Material for:

Platelets expressing IgG receptor FcγRIIA/CD32A determine the severity of experimental anaphylaxis

Héloïse Beutier, Béatrice Hechler, Ophélie Godon, Yu Wang, Caitlin M. Gillis, Luc de Chaisemartin, Aurélie Gouel-Ch?ron, Stéphanie Magnenat, Lynn E. Macdonald, Andrew J. Murphy, NASA study group, Sylvie Chollet-Martin, Dan Longrois, Christian Gachet, Pierre Bruhns,* Friederike Jönsson*

*Corresponding author. Email: joensson{at}pasteur.fr (F.J.); bruhns{at}pasteur.fr (P.B.)

Published 13 April 2018, Sci. Immunol. 3, eaan5997 (2018)
DOI: 10.1126/sciimmunol.aan5997

This PDF file includes:

  • NASA study group
  • Fig. S1. Clinical signs of HA-hIgG–dependent anaphylaxis and platelet depletion using anti-GPIbα antibodies.
  • Fig. S2. Platelets associate with Ly6Chi monocytes during HA-hIgG–dependent anaphylaxis and require FcγRIIA expression for activation.
  • Fig. S3. Serotonin but not histamine concentration is augmented during HAhIgG–dependent anaphylaxis.
  • Fig. S4. Generation of hFcγRKI mice.
  • Fig. S5. Expression of hFcγRs in blood of humans or transgenic mice.
  • Fig. S6. Formation of platelet/neutrophil aggregates is dispensable for HA-hIgG–dependent anaphylaxis in hFcγRKI mice.
  • Fig. S7. Platelet gating in NASA study.
  • Fig. S8. Platelet-bound hIgG and hFcγRIIA expression on platelets ex vivo after HA-hIgG injection into hFcγRIIAtg mice.

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Correction (03 October 2018): Figure S5C showed inverted annotations for the T and B cell panels. These have been corrected. There are no changes to data or conclusions.

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