Science Immunology

Supplementary Materials

Supplementary Material for:

Tumor suppressor BAP1 is essential for thymic development and proliferative responses of T lymphocytes

Teresita L. Arenzana, Steve Lianoglou, Akiko Seki, Celine Eidenschenk, Tommy Cheung, Dhaya Seshasayee, Thijs Hagenbeek, Arivazhagan Sambandam, Rajkumar Noubade, Ivan Peng, Justin Lesch, Jason DeVoss, Xiumin Wu, Wyne P. Lee, Patrick Caplazi, Joshua Webster, Jinfeng Liu, Victoria C. Pham, David Arnott, Jennie R. Lill, Zora Modrusan, Anwesha Dey,* Sascha Rutz*

*Corresponding author. Email: dey.anwesha{at}gene.com (A.D.); saschar{at}gene.com (S.R.)

Published 20 April 2018, Sci. Immunol. 3, eaal1953 (2018)
DOI: 10.1126/sciimmunol.aal1953

This PDF file includes:

  • Materials and Methods
  • Fig. S1. Loss of γδ T cells in the thymus of BAP1-deficient mice.
  • Fig. S2. Thymocyte defect in BAP1-deficient mice is intrinsic to the hematopoietic compartment.
  • Fig. S3. RNA expression levels of members of the PRCs in BAP1-deficient DN3 T cells.
  • Fig. S4. Loss of BAP1 in myeloid and B cell progenitors results in increase of global H2AK119ub levels.
  • Fig. S5. Tamoxifen-induced deletion of BAP1 results in reduced numbers of peripheral T cell populations.
  • Fig. S6. Thymocyte development is grossly normal in Bap1fl/flCd4-Cre+ mice.
  • Fig. S7. BAP1 deficiency impairs maintenance of peripheral T cells.
  • Fig. S8. BAP1 is not required for early TH cell differentiation.
  • Fig. S9. BAP1 deficiency impairs peripheral T cell proliferation and effector function.
  • Fig. S10. BAP1-deficient CD4 T cells have cell-intrinsic proliferative defect.
  • Fig. S11. Deubiquitinating activity, but not HCF-1 binding, is required for BAP1 function in peripheral T cells.
  • Table S2. Definition of cell populations and gating strategy.

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Other Supplementary Material for this manuscript includes the following:

  • Table S1 (Microsoft Excel format). Raw data sets.

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