Science Immunology

Supplementary Materials

Supplementary Material for:

Transcription factor ID2 prevents E proteins from enforcing a na?ve T lymphocyte gene program during NK cell development

Erin C. Zook, Zhong-Yin Li, Yiying Xu, Renée F. de Pooter, Mihalis Verykokakis, Aimee Beaulieu, Anna Lasorella, Mark Maienschein-Cline, Joseph C. Sun, Mikael Sigvardsson, Barbara L. Kee*

*Corresponding author. Email: bkee{at}bsd.uchicago.edu (A.D.)

Published 27 April 2018, Sci. Immunol. 3, eaao2139 (2018)
DOI: 10.1126/sciimmunol.aao2139

This PDF file includes:

  • Fig. S1. ID2 is required for the development of NK cells, liver ILC1, and BM ILC2.
  • Fig. S2. RId2-/- NK cells expand in vitro and phosphorylate S6 kinase similarly to Ctrl CD27+CD11b- NK cells.
  • Fig. S3. Activation of AP-1 restores IFN-γ production in RId2-/- NK cells.
  • Fig. S4. Comparison of differential chromatin accessibility indicates that NK cell maturation has partial parallels to a CD8 MP to effector transition.
  • Fig. S5. Cell-extrinsic deficiency of NK cells in Id3-/- mice caused by "γδNKTlike" cells.
  • Legends for tables S1 to S4

Download PDF

Other Supplementary Material for this manuscript includes the following:

  • Table S1 (Microsoft Excel format). Summary of gene set enrichment analysis for genes differentially expressed in Ctrl and RId2-/- CD27+CD11b- NK cells.
  • Table S2 (Microsoft Excel format). Metascape pathway analysis for genes differentially expressed in Ctrl and RId2-/- CD27+CD11b- NK cells.
  • Table S3 (Microsoft Excel format). Location of top five E box motifs in open chromatin with increased accessibility near genes with increased expression in RId2-/- NK cells.
  • Table S4 (Microsoft Excel format). Raw data for Figs. 1 to 4 and 7, and figs. S1 to S3 and S5.

Files in this Data Supplement: