Science Immunology

Supplementary Materials

Supplementary Material for:

MicroRNA-146a controls functional plasticity in γδ T cells by targeting NOD1

Nina Schmolka,* Pedro H. Papotto, Paula Vargas Romero, Tiago Amado, Francisco J. Enguita, Ana Amorim, Ana F. Rodrigues, Katrina E. Gordon, Ana S. Coroadinha, Mark Boldin, Karine Serre, Amy H. Buck, Anita Q. Gomes,* Bruno Silva-Santos*

*Corresponding author. Email: nina.schmolka{at}uzh.ch (N.S.); anitagomes{at}medicina.ulisboa.pt (A.Q.G.); bssantos{at}medicina.ulisboa.pt (B.S.-S.)

Published 4 May 2018, Sci. Immunol. 3, eaao1392 (2018)
DOI: 10.1126/sciimmunol.aao1392

This PDF file includes:

  • Fig. S1. miR-146a expression analysis.
  • Fig. S2. Loss of miR-146a does not affect steady-state γδ T cell subsets.
  • Fig. S3. Loss of miR-146a does not affect IFN-γ production by γδ27+ T cells or CD4+ TH1 cells upon in vitro polarization.
  • Fig. S4. Mixed BM and neonatal thymocyte chimeras.
  • Fig. S5. γδ T cell responses to L. monocytogenes infection.
  • Fig. S6. Differential Argonaute 2 immunoprecipitation for identification of mRNA targets of miR-146a.
  • Fig. S7. Nod1 is targeted by miR-146a in γδ T cells.
  • Fig. S8. Nod1 restriction does not affect IFN-γ production by γδ27+ T cells.
  • Table S1. Top 25 miscellaneous mRNAs differentially enriched upon Ago2-RNA immunoprecipitation in T cells overexpressing miR-146a.

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Other Supplementary Material for this manuscript includes the following:

  • Table S2 (Microsoft Excel format). Raw data sets.

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