Science Immunology

Supplementary Materials

Supplementary Material for:

Tumor immune evasion arises through loss of TNF sensitivity

Conor J. Kearney, Stephin J. Vervoort, Simon J. Hogg, Kelly M. Ramsbottom, Andrew J. Freeman, Najoua Lalaoui, Lizzy Pijpers, Jessica Michie, Kristin K. Brown, Deborah A. Knight, Vivien Sutton, Paul A. Beavis, Ilia Voskoboinik, Phil K. Darcy, John Silke, Joseph A. Trapani, Ricky W. Johnstone,* Jane Oliaro*

*Corresponding author. Email: ricky.johnstone{at}petermac.org (R.W.J.); jane.oliaro{at}petermac.org (J.O.)

Published 18 May 2018, Sci. Immunol. 3, eaar3451 (2017)
DOI: 10.1126/sciimmunol.aar3451

This PDF file includes:

  • Fig. S1. Additional validation and control for CRISPR screen.
  • Fig. S2. Confirmation of CRISPR gene deletion.
  • Fig. S3. Transcriptional analysis of cytokine-treated tumor cells.
  • Fig. S4. Additional cytokine treatment data.
  • Fig. S5. Further adoptive T cell therapy screen data.
  • Fig. S6. Validation of Ado knockout TNF response in additional tumor lines.
  • Fig. S7. Confirmation of Gosr1 as an immune evasion gene.
  • Legends for movies S1 and S2

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Other Supplementary Material for this manuscript includes the following:

  • Movie S1 (.mov format). Time-lapse imaging of T cells killing MC38Ova or MC38Vec cells.
  • Movie S2 (.mov format). Time-lapse imaging of T cells killing MC38Ova or MC38Vec cells in the presence of anti-TNF antibody.
  • Table S1 (Microsoft Excel format). Raw data.
  • Table S2 (Microsoft Excel format). CRISPR screen data, B16 perforin knockout OT-I screen.
  • Table S3 (Microsoft Excel format). CRISPR screen data, B16 OT-I screen.
  • Table S4 (Microsoft Excel format). CRISPR screen data, MC38 OT-I screen.
  • Table S5 (Microsoft Excel format). CRISPR screen data, MDA CAR T cell screen.
  • Table S6 (Microsoft Excel format). CRISPR screen data, OT-I IgG versus T0.
  • Table S7 (Microsoft Excel format). CRISPR screen data, OT-I PD-1 versus T0.
  • Table S8 (Microsoft Excel format). CRISPR screen data, MC38 NK cell.

Files in this Data Supplement: