Science Immunology

Supplementary Materials

Supplementary Material for:

Regulatory T cells induce activation rather than suppression of human basophils

Meenu Sharma, Mrinmoy Das, Emmanuel Stephen-Victor, Caroline Galeotti, Anupama Karnam, Mohan S. Maddur, Patrick Bruneval, Srini V. Kaveri, Jagadeesh Bayry*

*Corresponding author. Email: jagadeesh.bayry{at}crc.jussieu.fr

Published 25 May 2018, Sci. Immunol. 3, eaan0829 (2017)
DOI: 10.1126/sciimmunol.aan0829

This PDF file includes:

  • Materials and Methods
  • Fig. S1. Expression of HLA-DR, CD80, and CD86 on human DCs.
  • Fig. S2. Expression of TGF-βRII and IL-10Rα on human splenic basophils.
  • Fig. S3. Effect of IL-10 on the phenotype of human DCs.
  • Fig. S4. Anti-CD3 and anti-CD28 mAbs do not alter the phenotype of basophils.
  • Fig. S5. Blocking of ICAM-1 on DCs inhibits DC-mediated CD4+ T cell proliferation.
  • Fig. S6. ICOS-ICOSL interaction is dispensable for the Treg-mediated human basophil activation.
  • Fig. S7. Representative dot plots depicting the absence of CD4+ T cells in the lower chambers of transwells.
  • Fig. S8. IL-3 secretion by human Tregs.
  • Fig. S9. Dose-dependent effects of IL-3 on the phenotype of human basophils.
  • Fig. S10. Tregs license basophils to undergo activation by degranulation signals.
  • Fig. S11. The phenotypic and functional features of isolated human Tregs.
  • Fig. S12. The positive controls for annexin V and PI staining.

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Other Supplementary Material for this manuscript includes the following:

  • Table S1. Raw data from figure graphs (Excel).

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