Science Immunology

Supplementary Materials

Supplementary Material for:

Identification and characterization of HIV-specific resident memory CD8+ T cells in human lymphoid tissue

Marcus Buggert,* Son Nguyen, Gonzalo Salgado-Montes de Oca, Bertram Bengsch, Samuel Darko, Amy Ransier, Emily R. Roberts, Daniel del Alcazar, Irene Bukh Brody, Laura A. Vella, Lalit Beura, Sathi Wijeyesinghe, Ramin S. Herati, Perla M. Del Rio Estrada, Yuria Ablanedo-Terrazas, Leticia Kuri-Cervantes, Alberto Sada Japp, Sasikanth Manne, Shant Vartanian, Austin Huffman, Johan K. Sandberg, Emma Gostick, Gregory Nadolski, Guido Silvestri, David H. Canaday, David A. Price, Constantinos Petrovas, Laura F. Su, Golnaz Vahedi, Yoav Dori, Ian Frank, Maxim G. Itkin, E. John Wherry, Steven G. Deeks, Ali Naji, Gustavo Reyes-Terán, David Masopust, Daniel C. Douek, Michael R. Betts*

*Corresponding authors. Email: betts{at}pennmedicine.upenn.edu (M.R.B.); marcus.buggert{at}ki.se (M.B.)

Published 1 June 2018, Sci. Immunol. 3, eaar4526 (2017)
DOI: 10.1126/sciimmunol.aar4526

This PDF file includes:

  • Materials and Methods
  • Fig. S1. Maturation of and cycling of LN CD8+ T cells.
  • Fig. S2. CD69 expression on naïve CD8+ T cells and immune activation profile of CD69 and CD69+ CD8+ T cells in LTs.
  • Fig. S3. Phenotypic characteristics of memory CD8+ T cells in blood and LTs.
  • Fig. S4. Residency characteristics of CD69 and CD69+ CD8+ T cells.
  • Fig. S5. RNA-seq, ATAC-seq, and CCR7 analysis of LN TRMs.
  • Fig. S6. Imaging analysis and CD103 expression in TDL.
  • Fig. S7. Bulk and HIV-specific analysis and rhesus gating strategy.
  • Fig. S8. Index sorting strategy and scRNA-seq analysis.
  • Fig. S9. scRNA-seq analysis of LN HIV-specific CD8+ T cells.
  • Table S1. Cohort characteristics.
  • Table S2. List of significant genes up-regulated/down-regulated from RNA-seq.
  • Table S3. List of significant ATAC-seq tracks.
  • Table S4. List of top 200 significant genes up-regulated/down-regulated from scRNA-seq.

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