Science Immunology

Supplementary Materials

Supplementary Material for:

CD4+ T cell–mediated HLA class II cross-restriction in HIV controllers

Moran Galperin, Carine Farenc, Madhura Mukhopadhyay, Dhilshan Jayasinghe, Amandine Decroos, Daniela Benati, Li Lynn Tan, Lisa Ciacchi, Hugh H. Reid, Jamie Rossjohn*, Lisa A. Chakrabarti*, Stephanie Gras*

*Corresponding authors. Email: jamie.rossjohn{at} (J.R.); chakra{at} (L.A.C.); stephanie.gras{at} (S.G.)

Published 8 June 2018, Sci. Immunol. 3, eaat0687 (2018)
DOI: 10.1126/sciimmunol.aat0687

This PDF file includes:

  • Materials and Methods
  • Fig. S1. HLA-DR polymorphism.
  • Fig. S2. Representative TCR binding curves determined by SPR across different HLA-DR molecules.
  • Fig. S3. Gating strategies and masks used for the identification of cellular conjugates and immunological synapses.
  • Fig. S4. Representative example of TCR transduction efficiency in human peripheral blood mononuclear cells from a healthy donor.
  • Fig. S5. Gating strategy used for the single-cycle viral inhibition assay.
  • Fig. S6. Correlation between cytotoxic phenotype and viral inhibition.
  • Fig. S7. TCR-mediated elimination of HIV-infected DCs.
  • Fig. S8. Omit maps and refined maps of the peptide–HLA-DR complexes.
  • Fig. S9. Impact of HLA-DR polymorphism on epitope presentation and antigenbinding cleft conformation.
  • Fig. S10. The F24 and F5 TCRs engage with the HLA-DR11–RQ13 in the same fashion.
  • Fig. S11. Structural changes in the TCRs are limited to the CDR3β loop.
  • Fig. S12. Representative binding curves determined by SPR for HLA-DR11 mutants.
  • Table S1. List of peptides used in the study.
  • Table S2. Data collection and refinement statistics of peptide–HLA-DR structures.
  • Table S3. Data collection and refinement statistics of TCR peptide–HLA-DR structures.
  • Table S4. Contact table of F24 TCR–HLA-DR11–RQ13.
  • Table S5. Contact table of F24 TCR–HLA-DR15–RQ13.
  • Table S6. Contact table of F24 TCR–HLA-DR1–RQ13.
  • Table S7. Data collection and refinement statistics of F24 TCR structure.
  • Table S8. Energetic contribution of the HLA-DR11 residues as measured by SPR.
  • Table S9. Affinity measurement of the F24 TCR mutants.
  • References (30–32)

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