Science Immunology

Supplementary Materials

The PDF file includes:

  • Materials and Methods
  • Fig. S1. Creation and stimulation of hybridoma T cell lines with NFAT and NFκB reporters.
  • Fig. S2. Stimulation and characterization of C7 and C26 transgenic CD4+ T cells in vitro.
  • Fig. S3. Analysis of CD25 expression in an endogenous CD4+ T cell repertoire early after viral infection.
  • Fig. S4. Characterization of C7 and C26 transgenic CD4+ T cells via adoptive transfer and LCMV infection.
  • Fig. S5. Early expression of CD25 by clone 18 retrogenic CD4+ T cells predicts effector and memory differentiation.
  • Fig. S6. SHP-1 KD validation and effect on TCR signaling in vitro.
  • Fig. S7. Flow cytometry gating strategies.

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Other Supplementary Material for this manuscript includes the following:

  • Table S1 (Microsoft Excel format). Primary source data.
  • Table S2 (Microsoft Excel format). Complete list of genes with significantly changed expression when comparing CD25lo to CD25hi day 5 SMARTA effector cells, as determined by RNA-seq.

Files in this Data Supplement: