Science Immunology

Supplementary Materials

The PDF file includes:

  • Fig. S1. A mouse model of neutrophilic airway inflammation is associated with the neutrophil recruitment and TH17 activation during sensitization.
  • Fig. S2. Gating strategy to identify neutrophils and cytoplasts.
  • Fig. S3. Antiā€“IL-17 administration during the sensitization phase blunts neutrophilia without affecting cytoplast generation.
  • Fig. S4. Protease-free DNase does not affect neutrophilia.
  • Fig. S5. Citrullinated histone identified on neutrophils from WT mice.
  • Fig. S6. Cytoplast promotes T cell responses via contact-dependent mechanism.
  • Fig. S7. Gating strategies for detection of cytoplasts in human BALF from severe asthmatics.
  • Table S1. Patient characteristics.
  • Table S2. Neutrophil and DNA high asthma patient symptoms, lung function, and comorbidities.
  • Legends for movies S1 and S2

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Other Supplementary Material for this manuscript includes the following:

  • Movie S1 (.avi format). Murine lung neutrophils were flow-sorted after HDM/LPS treatment (3-day exposure), and chemotaxis to LTB4 was monitored in a microfluidic chamber.
  • Movie S2 (.avi format). Murine lung cytoplasts were flow-sorted after HDM/LPS treatment (3-day exposure), and chemotaxis to LTB4 was monitored in a microfluidic chamber.
  • Raw data (Microsoft Excel format)

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