Science Immunology

Supplementary Materials

The PDF file includes:

  • Fig. S1. GSDMD mediates rapid macrophage pyroptosis.
  • Fig. S2. Salmonella Typhimurium and nigericin induce IL-1β secretion from WT neutrophils.
  • Fig. S3. Cytosolic LPS triggers caspase-11–dependent cell death and IL-1β production from macrophages.
  • Fig. S4. Extracellular potassium blocks noncanonical NLRP3 activation and resultant release of mature IL-1β.
  • Fig. S5. Cytosolic LPS but not mock or flagellin transfection triggers GSDMD-dependent cell death.
  • Fig. S6. Time course analysis profiles the kinetics of caspase-11/GSDMD-induced NETosis.
  • Fig. S7. NE, MPO, and PAD4 are dispensable for GSDMD cleavage and caspase-11–driven NETosis.
  • Fig. S8. PMA induces NETosis of human neutrophils.
  • Fig. S9. Cytosolic bacteria triggers caspase-11–dependent NETs in vivo.
  • Fig. S10. Model for LPS-driven NETosis via GSDMD during cytosolic infection.

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Other Supplementary Material for this manuscript includes the following:

  • Table S1 (Microsoft Excel format). Raw data file.

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