Science Immunology

The PDF file includes:

• Materials and Methods
• Fig. S1. Screening strategy and titration curves.
• Fig. S2. NET-inhibiting compound LDC7559 and its derivative bind to GSDMD.
• Fig. S3. GSDMD cleavage and localization during NET formation.
• Fig. S4. TIRF microscopy of NE during NET formation.
• Fig. S5. NE processes GSDMD.
• Fig. S6. Mode of action of LDC7559.
• Fig. S7. Live-cell imaging of NET formation.
• Fig. S8. Model of GSDMD involvement in NET formation.
• Legends for movies S1 to S4
• References (32–34)

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Other Supplementary Material for this manuscript includes the following:

• Movie S1 (.avi format). Classification of cell states during NET formation.
• Movie S2 (.avi format). PMA-induced NET formation.
• Movie S3 (.avi format). PMA-induced NET formation in the presence of LDC7559.
• Movie S4 (.avi format). Nigericin-induced NET formation.
• Movie S5 (.avi format). Nigericin-induced NET formation in the presence of LDC7559.
• Table S1 (Microsoft Excel format). Raw data.