Science Immunology
Supplementary Materials
The PDF file includes:
- Materials and Methods
- Fig. S1. Screening strategy and titration curves.
- Fig. S2. NET-inhibiting compound LDC7559 and its derivative bind to GSDMD.
- Fig. S3. GSDMD cleavage and localization during NET formation.
- Fig. S4. TIRF microscopy of NE during NET formation.
- Fig. S5. NE processes GSDMD.
- Fig. S6. Mode of action of LDC7559.
- Fig. S7. Live-cell imaging of NET formation.
- Fig. S8. Model of GSDMD involvement in NET formation.
- Legends for movies S1 to S4
- References (32–34)
Other Supplementary Material for this manuscript includes the following:
- Movie S1 (.avi format). Classification of cell states during NET formation.
- Movie S2 (.avi format). PMA-induced NET formation.
- Movie S3 (.avi format). PMA-induced NET formation in the presence of LDC7559.
- Movie S4 (.avi format). Nigericin-induced NET formation.
- Movie S5 (.avi format). Nigericin-induced NET formation in the presence of LDC7559.
- Table S1 (Microsoft Excel format). Raw data.
Files in this Data Supplement: