Science Immunology

Supplementary Materials

The PDF file includes:

  • Fig. S1. Lymphoma B cells developing in Eμ-myc transgenic mice display extensive intratumor genetic diversity.
  • Fig. S2. Frequent chromosome loss in MYC-driven lymphoma B cells.
  • Fig. S3. Multicolor fluorescently labeled tumor cells maintain clonal and genomic heterogeneity.
  • Fig. S4. Tumor heterogeneity is also detected in the blood.
  • Fig. S5. Tumor clonal dominance in nonlymphoid sites is independent of the adaptive immune system.
  • Fig. S6. Tumor-specific CD8+ T cells form long-lasting contacts with tumor cells in the bone marrow.
  • Fig. S7. Restricted tumor diversity in female recipients as detected by intravital imaging.
  • Fig. S8. The immune-mediated restriction of intratumor heterogeneity is not reversible upon in vitro culture.
  • Fig. S9. Untransduced lymphoma cells exhibit higher phenotypic diversity in males compared with females.
  • Fig. S10. The restriction of tumor heterogeneity requires the adaptive immune system with an important role for CD8+ T cells.
  • Fig. S11. Lymphoma cells with an X-only karyotype are less diverse in females compared with males.
  • Fig. S12. GFP is not more immunogenic in female than in male mice.
  • Fig. S13. Putative neoantigens in Eμ-myc tumors.
  • Fig. S14. Modest reduction in tumor load upon checkpoint blockade.
  • Fig. S15. Restriction of tumor diversity mediated by checkpoint blockade is associated with tumor immunoediting.
  • Legends of movies S1 to S7

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Other Supplementary Material for this manuscript includes the following:

  • Movies S1 and S2 (.mov format). Lymphoma subclones establish independent niches in the bone marrow.
  • Movie S3 (.mov format). CD8+ T cells infiltrate the bone marrow and interact with lymphoma cells.
  • Movie S4 (.mov format). CD8+ T cells form stable contacts with lymphoma cells.
  • Movie S5 (.avi format). Tumor-reactive but not control CD8+ T cells establish stable contacts with tumor cells in the bone marrow.
  • Movie S6 (.avi format). Visualization of lymphoma cell apoptosis after interactions with tumor-reactive CD8+ T cells.
  • Movie S7 (.avi format). Lymphoma cell apoptosis after interactions with tumor-reactive CD8+ T cells but not control T cells.
  • Table S1. Raw datasets.

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