Science Immunology

Supplementary Materials

The PDF file includes:

  • Methods
  • Fig. S1. Fluorescence-activated cell sorting gating strategy for naïve, memory, LZ GC, and DZ GC B cells.
  • Fig. S2. Quantification of dynamic movement of unique pod-like structures formed in the immune synapse of LZ GC B cell and expression level of BCR and ezrin.
  • Fig. S3. Antigen-induced BCR signaling in naïve B cells and GC B cells.
  • Fig. S4. Design of force sensor and detection of pod-like structures.
  • Fig. S5. Polarization of SNX9 and SNX18 in naïve and LZ GC B cells after activation on antigen-containing PMS.
  • Fig. S6. The differential expression of key surface markers.
  • Fig. S7. Surface expression of LFA-1 and specificity of VLA-4 binding.
  • Legends for movies S1 to S10
  • Legend for table S1

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Other Supplementary Material for this manuscript includes the following:

  • Movie S1 (.mov format). Distinctive membrane dynamics in the immune synapse of live naïve and LZ GC B cells placed on antigen-containing PLB were visualized by DIC and IRM.
  • Movie S2 (.mov format). The dynamics of membrane movement in the immune synapse upon activation were visualized by IRM.
  • Movie S3 (.mov format). Time-lapse imaging of CD19 and BCR in the immune synapse of naïve B cells and LZ GC B cells.
  • Movie S4 (.mov format). Pulling forces in the immune synapse of live naïve and LZ GC B cells were monitored with time using DNA-based force sensors.
  • Movie S5 (.mov format). Colocalization of the cell contact sites and the locations of pulling force in LZ GC B cell.
  • Movie S6 (.mov format). The dynamics of membrane movement in the immune synapse of naïve and LZ GC B cell upon activation by high- or low-affinity antigen were visualized by DIC and IRM.
  • Movie S7 (.mov format). Motile LZ GC B cells were observed upon activation by low-affinity antigen.
  • Movie S8 (.mov format). Distinct antigen transport pattern shown in human LZ GC B cells on PMS presenting surrogate antigens as compared with naïve B cells.
  • Movie S9 (.mov format). Localization of MTOC with SNX9 or SNX18 after activation in naïve and LZ GC B cells.
  • Movie S10 (.mov format). Localization of LAMP-1 after activation in naïve and LZ GC B cells.
  • Table S1. Raw data file (Excel file).

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