Science Immunology

Supplementary Materials

The PDF file includes:

  • Methods
  • Fig. S1. Candidate transcription factors to instruct DC cell fate.
  • Fig. S2. Clec9a-based reporter to identify transcription factors to induce DC fate.
  • Fig. S3. Screening for transcription factors to activate Clec9a-tdT.
  • Fig. S4. DC morphology and dendrites are established in mouse fibroblasts.
  • Fig. S5. Spi1, Irf8, and Batf3 are enriched in cDC1 cells.
  • Fig. S6. Global single-cell gene expression analysis during iDC reprogramming.
  • Fig. S7. Stepwise transitions during iDC reprogramming.
  • Fig. S8. iDC reprogramming does not induce alterations associated with tumorigenesis.
  • Fig. S9. Analysis of iDC maturation states.
  • Fig. S10. Induced DCs are functional APCs.
  • Fig. S11. Polycistronic vector induces cDC1-like transcriptional program.
  • Fig. S12. PIB induce human iDC reprogramming.
  • Fig. S13. FACS gating strategies.
  • Legends for tables S1 to S5
  • Legends for movies S1 and S2
  • References (53–55)

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Other Supplementary Material for this manuscript includes the following:

  • Table S1 (Microsoft Excel format). Analysis of candidate transcription factors.
  • Table S2 (Microsoft Excel format). Single-cell mRNA-seq data analysis.
  • Table S3 (Microsoft Excel format). Pseudotime single-cell mRNA-seq data analysis.
  • Table S4 (Microsoft Excel format). Population mRNA-seq data analysis.
  • Table S5 (Microsoft Excel format). Raw data.
  • Movie S1 (.avi format). Time lapse showing Clec9a-tdT+ cell emergence.
  • Movie S2 (.mp4 format). Time lapse displaying dead cell incorporation.

Files in this Data Supplement: