Science Immunology

Supplementary Materials

This PDF file includes:

  • Materials and Methods
  • Fig. S1. Generation of human CD8 T cells from cord blood OP9-DL4 cell cocultures.
  • Fig. S2. Generation of human MART-1+ γδ TCR T cells from cord blood HSPCs cocultured with OP9-DL4 cells.
  • Fig. S3. Generation of human MART-1+ γδ TCR cells and clones from cord blood HSPCs cocultured with OP9-DL4 cells.
  • Fig. S4. Derivation of human MART-1+ γδ TCR T cells from adult PBMCs.
  • Fig. S5. Derivation of human MART-1+ γδ TCR T cells from cord blood naïve PBMCs.
  • Fig. S6. Derivation of human MART-1+ γδ TCR T cell line from pooled cord blood naïve PBMCs.
  • Fig. S7. Derivation of human MART-1+ γδ TCR T cell clones from individual cord blood PBMCs.
  • Fig. S8. Functional characterization of MART-1+ γδ TCR T cell clones generated from cord blood HSPCs cocultured on OP9-DL4 cells and from MART-1+ γδ TCR T cell lines derived from cord blood naïve PBMCs.
  • Fig. S9. Specificity of γδ TCR–transduced J76CD8α T cells.
  • Fig. S10. Cold target inhibition of γδ TCR staining by HLA/peptide dextramers.
  • Fig. S11. Functional characterization of TCR-transduced J76 T cells.
  • Fig. S12. Transduction and isolation of PBMCs ectopically expressing 3C2 γδ TCR.
  • Fig. S13. Binding analysis of human γδ TCRs to MHCp using BLI.
  • Fig. S14. Contribution of germline and recombined residues of the 5F3 γδ TCR in recognition of HLA-A*02/MART-1.
  • Table S1. Generation of γδ TCR CD8α+ T cells clones with specificity for HLA-A*0201/MART-1 from HSPC/OP9-DL4 cell cocultures.
  • Table S2. Derivation of γδ TCR CD8α+ T cells clones with specificity for HLA-A*0201/MART-1 from naïve PBMCs.
  • Table S3. Cloning and sequencing of three in vitro–derived γδ TCR T cell clones.
  • Table S4. Data collection and refinement statistics of HLA-A*02-MART-1/5F3 TCR complex.
  • Table S5. Contacts residues between γδ TCR and HLA-A*02.
  • Table S6. Percentage of BSA contribution of complex components.

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