Science Immunology

Supplementary Materials

The PDF file includes:

  • Materials and Methods
  • Fig. S1. Visualization of nuclear and cytoplasmic actin dynamics upon TCR stimulation.
  • Fig. S2. Calcium and actin dynamics during T cell stimulation.
  • Fig. S3. Screen for actin nucleators involved in nuclear actin polymerization upon T cell stimulation.
  • Fig. S4. Effect of CK-869 on Ca2+ and actin dynamics.
  • Fig. S5. Effect of T cell activation on subcellular distribution of Arp3.
  • Fig. S6. Influence of nuc.dnArp2.mCherry on actin and Ca2+ dynamics upon T cell stimulation.
  • Fig. S7. Cytokine release upon T cell stimulation in the presence of CK-869.
  • Fig. S8. Differentially expressed genes upon T cell stimulation and inhibition of Arp2/3 or nuclear calcium.
  • Fig. S9. Gene expression of cytokines is affected early after activation in the presence of CK-869.
  • Fig. S10. In vivo model system used for analysis of T cell help.
  • Legends for tables S1 and S2
  • Legends for movies S1 to S10
  • References (4352)

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Other Supplementary Material for this manuscript includes the following:

  • Table S1 (Microsoft Excel format). Time-dependent differential gene expression of primary human CD4+ T cells upon PMA/Iono activation and CK-869 treatment.
  • Table S2 (Microsoft Excel format). Raw data of all figure graphs.
  • Movie S1 (.mov format). Nuclear actin polymerization precedes formation of circumferential F-actin rings at the PM triggered by TCR engagement.
  • Movie S2 (.mov format). Nuclear actin polymerization upon immune synapse formation.
  • Movie S3 (.mov format). TCR signaling induces nuclear actin polymerization.
  • Movie S4 (.mov format). Calcium release and nuclear actin polymerization coincide.
  • Movie S5 (.mov format). Inhibition of nuclear calcium signaling inhibits nuclear actin polymerization.
  • Movie S6 (.mov format). Three different shRNAs targeting Arp3 expressed in Jurkat NLA impair formation of PMA/Iono-induced nuclear actin filaments.
  • Movie S7 (.mov format). Pharmacological inhibition of Arp2/3 complex impairs formation of TCR-induced nuclear actin filaments.
  • Movie S8 (.mov format). Inhibition of nuclear Arp2/3 impairs formation of TCR-induced nuclear actin filaments.
  • Movie S9 (.mov format). Specific inhibition of nuclear Arp2/3 and the effect on nuclear actin polymerization on immune synapse formation in T cells.
  • Movie S10 (.mov format). Pharmacological inhibition of Arp2/3 inhibits nuclear actin polymerization in primary T cells.

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