Science Immunology

Supplementary Materials

The PDF file includes:

  • Materials and Methods
  • Fig. S1. Comprehensive epitope mapping strategy and experimental workflow.
  • Fig. S2. Quality and detection of antigen-specific CD8+ T cells using highly multiplexed combinatorial pMHC tetramer staining and mass cytometry.
  • Fig. S3. Overall magnitudes of antigen-specific CD8+ T cell response in various clinical stages during HBV infection.
  • Fig. S4. Validation of highly multiplexed combinatorial pMHC tetramer strategy in HLA-A*11:01 and non–HLA-A*11:01 donors.
  • Fig. S5. Validation and reproducibility of antigen-specific CD8+ T cells using flow cytometry and the serological measurement of HDs.
  • Fig. S6. In vitro expansion of antigen-specific CD8+ T cells upon peptide stimulation.
  • Fig. S7. Expression levels of nine different inhibitory receptors on antigen-specific CD8+ T cells.
  • Fig. S8. Epitope frequencies in longitudinal patient cohorts of HBeAg seroconverters.
  • Fig. S9. Cellular profiles of subset clusters of HBV-specific CD8+ T cells identified by Phenograph and the enrichment strategy.
  • Fig. S10. Unsupervised Phenograph clustering analysis identified multifactorial T cell heterogeneity of HBV-specific CD8+ T cells.
  • Fig. S11. Coexpression of inhibitory receptors on virus-specific CD8+ T cells.
  • Fig. S12. Heterogeneous multifunctional subsets of virus-specific CD8+ T cells.
  • Fig. S13. Diverse characteristics of epitope-specific TCRβ repertoire using TCRdist.
  • Fig. S14. Dynamics of cellular response and viral mutation of HBVcore169-specific CD8+ T cells in a longitudinal patient cohort.
  • Fig. S15. Staining quality of cellular markers including nine inhibitory receptors using mass cytometry.
  • Table S1. List of screened HLA-A*11:01–restricted epitopes and the detected frequency.
  • Table S2. List of the antibody staining panels used for mass cytometry and high-dimensional cytometric data analysis.
  • Table S3. List of the patient samples and the clinical and serological information.
  • Table S4. Frequency of viral mutation on selective epitopes in longitudinal patient cohort across HBeAg seroconversion.
  • Legend for movie S1
  • References (7279)

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Other Supplementary Material for this manuscript includes the following:

  • Movie S1 (.mov format). 3D image of categorical analysis of HBV-specific CD8+ T cells using One-SENSE.

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