Science Immunology

Supplementary Materials

The PDF file includes:

  • Supplementary Methods
  • Fig. S1. Staining for Ki67 in cDCs and their progenitors.
  • Fig. S2. Flow cytometric analysis of Clec9aConfetti organs.
  • Fig. S3. Analysis of Clec9aCre-based fate-mapping mice on a Flt3l−/− background.
  • Fig. S4. ClusterQuant 3D analysis of single-color cDC clustering in Clec9aConfetti mice.
  • Fig. S5. Analysis of Confetti+ cDC subsets.
  • Fig. S6. Quantification of infected cells and cell infiltration during influenza A virus infection.
  • Fig. S7. Voronoi diagrams of analyzed high and low infiltrated areas from infected Clec9aConfetti mice.
  • Fig. S8. Cluster size distribution in high and low infiltrated areas of lungs from infected Clec9aConfetti mice.
  • Fig. S9. Quantification of cDC proliferation and pre-cDC composition during influenza A virus infection.
  • Fig. S10. Flow cytometry gating strategy for CDPs.
  • Fig. S11. Flow cytometry gating strategy for cDC subsets and macrophages.
  • Fig. S12. Flow cytometry gating strategy for Confetti+ cells in Clec9aConfetti mice.
  • Legend for table S1
  • Table S2. Abs used in flow cytometry.
  • Table S3. Abs used in confocal microscopy.
  • Legends for movies S1 to S11
  • References (47, 48)

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Other Supplementary Material for this manuscript includes the following:

  • Table S1 (Microsoft Excel format). Excel file with raw data used to generate all graphs that have n < 25.
  • Movie S1 (.avi format). SI from a Clec9aConfetti mouse.
  • Movie S2 (.avi format). Lung from a Clec9aConfetti mouse.
  • Movie S3 (.avi format). SI from a Clec9aConfetti mouse crossed to a Flt3l−/− background.
  • Movie S4 (.avi format). Lung from a Clec9aConfetti mouse crossed to a Flt3l−/− background.
  • Movie S5 (.avi format). Representative annotated image of the SI of a Clec9aConfetti mouse.
  • Movie S6 (.avi format). Representative annotated imaged of the lung of a Clec9aConfetti mouse.
  • Movie S7 (.avi format). SI from a Clec9aConfetti mouse stained for cDC subsets.
  • Movie S8 (.avi format). Lung from a Clec9aConfetti mouse stained for cDC subsets.
  • Movie S9 (.avi format). Image an area of the lung of a Clec9aConfetti 1 dpi with influenza A virus and stained for viral proteins.
  • Movie S10 (.avi format). Representative annotated imaged of a high infiltrated area of the lung of a Clec9aConfetti 7 dpi with influenza A virus.
  • Movie S11 (.avi format). Representative annotated imaged of a low infiltrated area of the lung of a Clec9aConfetti 7 dpi with influenza A virus.

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