Science Immunology

Supplementary Materials

The PDF file includes:

  • Methods
  • Fig. S1. Gating strategy for donor and recipient T cells from BAL samples.
  • Fig. S2. Gating strategy for donor and recipient T cells from blood.
  • Fig. S3. Lung transplant recipients have low frequencies of CD4+ Tregs in blood and BAL samples.
  • Fig. S4. Single-cell RNA sequencing schematic and comparison between patients.
  • Fig. S5. Lack of association of donor T cell chimerism in BAL with viral infection.
  • Table S1. Patient demographics and transplant characteristics.
  • Table S2. Patient demographics for transplant recipients used for CD4+ Treg data.
  • Table S3. Summary of scRNA-seq results by a 96-well plate.
  • Table S4. Characteristics of the organ donors for control lung samples.
  • Table S5. List of antibodies used for flow cytometry and immunofluorescence imaging.
  • Table S6. Details of reverse transcriptase primers.
  • Table S7. Cell filtration cutoffs for P19.

Download PDF

Files in this Data Supplement: