Science Immunology

Supplementary Materials

The PDF file includes:

  • Materials and Methods
  • Fig. S1. F-actin accumulates at the leading edges of synaptic protrusions.
  • Fig. S2. Centralization factor analysis.
  • Fig. S3. PTEN depletion enhances F-actin accumulation in protrusions.
  • Fig. S4. Effects of CK666 on T cell activation.
  • Fig. S5. WASP and WAVE2 depletion induce distinct functional phenotypes.
  • Fig. S6. CTLs physically manipulate the target cell surface.
  • Legends for movies S1 to S17

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Other Supplementary Material for this manuscript includes the following:

  • Table S1 (Microsoft Excel format). Data used to generate all graphs.
  • Movie S1 (.mp4 format). CTLs form dynamic F-actin–rich protrusions on stimulatory micropillars.
  • Movie S2 (.mp4 format). Granule fusion occurs at the base of synaptic protrusions.
  • Movie S3 (.mp4 format). Protrusion formation by vehicle-treated CTLs.
  • Movie S4 (.mp4 format). Protrusion formation by CK666-treated CTLs.
  • Movie S5 (.mp4 format). Force exertion by vehicle-treated CTLs.
  • Movie S6 (.mp4 format). Force exertion by CK666-treated CTLs.
  • Movie S7 (.mp4 format). WAVE2-GFP accumulates in peripheral synaptic protrusions.
  • Movie S8 (.mp4 format). WASP-GFP accumulates in central synaptic protrusions.
  • Movie S9 (.mp4 format). Protrusive behavior of NT-CR CTLs.
  • Movie S10 (.mp4 format). Protrusive behavior of WASP-CR CTLs.
  • Movie S11 (.mp4 format). Protrusive behavior of WAVE2-CR CTLs.
  • Movie S12 (.mp4 format). Force exertion by NT-CR CTLs.
  • Movie S13 (.mp4 format). Force exertion by WASP-CR CTLs.
  • Movie S14 (.mp4 format). Force exertion by WAVE2-CR CTLs.
  • Movie S15 (.mp4 format). Target cell manipulation by NT-CR CTLs.
  • Movie S16 (.mp4 format). Target cell manipulation by WASP-CR CTLs.
  • Movie S17 (.mp4 format). Target cell manipulation by WAVE2-CR CTLs.

Files in this Data Supplement: