Science Immunology

Supplementary Materials

The PDF file includes:

  • Material and Methods
  • Fig. S1. Stimulation of P14 CD8+ T cells on α-CD3–, α-CD28–, and Fc-ICAM-1–coated plates or in a coculture with dendritic cells leads to comparable ACD rates.
  • Fig. S2. Transient mTOR inhibition was obtained by treatment with rapamycin or Akt-kinase inhibitor.
  • Fig. S3. Transient mTOR inhibition modulates T-bet asymmetry.
  • Fig. S4. Transient but not continuous mTOR inhibition is a requirement for ACD modulation in CD8+ T cells.
  • Fig. S5. Decreased ACD rates in P14 CD8+ T cells submitted to FTY720 treatment led to reduced proliferation upon LCMV challenge.
  • Fig. S6. CM CD8+ T cells produce progenies with enhanced memory potential after enforcing ACD.
  • Fig. S7. Murine and human rapamycin-treated naïve CD8+ T cells produce progenies that can survive better in the presence of limited amounts of IL-15.
  • Fig. S8. CD8lo and CD8hi daughter cells inherit distinct cyclin expression profiles.
  • Fig. S9. ACD generates progenies with strengthened memory signatures.
  • References (4755)

Download PDF

Other Supplementary Material for this manuscript includes the following:

  • Table S1 (Microsoft Excel format). Raw data files.

Files in this Data Supplement: