Science Immunology

Supplementary Materials

The PDF file includes:

  • Materials and Methods
  • Fig. S1. Influence of vaccination route and dose on H5 antibody responses.
  • Fig. S2. H1H5- or H5-specific antibody responses in PVEI, MN, and HAI assays.
  • Fig. S3. ELISA binding of H1H5- or H5-specific monoclonal antibodies to group 1 or 2 influenza A or influenza B (Massachusetts/2/12) HA proteins.
  • Fig. S4. Recognition of HA by IGHV3-30–derived antibodies.
  • Fig. S5. ELISA binding of H1H5- or H5-specific monoclonal antibodies isolated before vaccination to H5 Vietnam or H1 New Caledonia HA proteins.
  • Fig. S6. Increase in affinity of H5-specific antibodies and avidity maturation of sera against HA1 or HA2 subunits.
  • Fig. S7. Alignment of antibody clones that are members of three previously described multidonor classes of HA-specific broadly neutralizing antibodies.
  • Fig. S8. SHM of H1-specific antibodies over time.
  • Fig. S9. Gating strategy for analyzing and sorting.
  • Table S1. Serum neutralization of multiple influenza strains in the PVEI assay after Ad4-H5-Vtn vaccination.
  • Table S2. Neutralizing activity of monoclonal antibodies isolated from vaccinees against a five-virus panel.
  • Table S3. Crystallographic data collection and refinement statistics.
  • Table S4. Composition of 429 B01 epitope and paratope.
  • Table S5. Hydrogen bonds between HA and 429 B01 antibody.
  • Table S6. Virus strain abbreviations.
  • References (3746)

Download PDF

Other Supplementary Material for this manuscript includes the following:

Files in this Data Supplement: