Science Immunology

Supplementary Materials

The PDF file includes:

  • Materials and Methods
  • Fig. S1. Controls for cytofluorimetric assessment of IL-33 expression.
  • Fig. S2. IL-33 expression primarily by mSCs is a common feature across male fat depots.
  • Fig. S3. Pdgfra-driven Il33 ablation affects the amount of mature VAT Tregs without affecting body, fat depot weights, or VmSC quantities in young lean male mice.
  • Fig. S4. Intracellular anti–IL-33 staining is an alternative to IL-33 reporter fluorescence for VmSC subtype quantification.
  • Fig. S5. Distinct gene expression profiles of VmSC subsets reveal functional heterogeneity.
  • Fig. S6. Aging affects VAT Tregs, weights, and VmSCs.
  • Fig. S7. Gender-specific differences in Tregs.
  • Fig. S8. VmSCs constitute the main source of IL-33 in different adipose depot in females.
  • Fig. S9. Diet-induced obesity provokes VAT Treg loss and VmSC remodeling.
  • Fig. S10. Specific IL-33 sensing by VAT Tregs alters VmSC dynamics.
  • Legends for tables S1 to S6
  • References (3746)

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Other Supplementary Material for this manuscript includes the following:

  • Table S1 (Microsoft Excel format). Specific and shared gene clusters reveal VmSC subsets’ heterogeneity.
  • Table S2 (Microsoft Excel format). Overrepresented pathways of VmSC subsets from 8-week-old male mice.
  • Table S3 (Microsoft Excel format). Cardinal gene expression profiles in VmSC subsets.
  • Table S4 (Microsoft Excel format). Differential expression of extracellular matrix and soluble protein on VmSC subtypes.
  • Table S5 (Microsoft Excel format). GSEA of VmSC subsets from female vis-à-vis male mice aged 8 to 10 weeks.
  • Table S6 (Microsoft Excel format). Raw data.

Files in this Data Supplement: