Science Immunology

Supplementary Materials

The PDF file includes:

  • Fig. S1. C5aR2 contributes to the pathogenesis of IC-induced arthritis.
  • Fig. S2. ACKR1 contributes to the pathogenesis of IC-induced arthritis.
  • Fig. S3. C5aR2 expression on joint endothelium.
  • Fig. S4. In vivo superfusion assays.
  • Fig. S5. Immunofluorescence analysis of C5a expression on joint tissue harvested from WT and C5ar2-KO mice on day 7 after AST.
  • Fig. S6. In vivo superfusion assay using AF594-C5a.
  • Fig. S7. Analysis of CXCL1 transport into the lumen of joint blood vessels in WT, C5ar2-KO, and Ackr1-KO mice.
  • Fig. S8. Representative gating strategy of Ly6G+ neutrophil.
  • Legends for movies S1 to S7

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Other Supplementary Material for this manuscript includes the following:

  • Movie S1 (.mp4 format). In vivo MP-IVM joint imaging of live WT, C5ar2-KO, and Ackr1-KO BMC mice on day 3 after AST.
  • Movie S2 (.mp4 format). In vivo MP-IVM joint imaging of live WT, C5ar2-KO, and Ackr1-KO BMC mice on day 7 after AST.
  • Movie S3 (.mp4 format). In vivo superfusion assay (application of C5a).
  • Movie S4 (.mp4 format). In vivo superfusion assay (application of CXCL1).
  • Movie S5 (.mp4 format). In vivo superfusion assay (application of CXCL2).
  • Movie S6 (.mp4 format). Sequential in vivo superfusion assay (application of C5a followed by application of CXCL1, CXCL2, or LTB4).
  • Movie S7 (.mp4 format). Sequential in vivo superfusion assay (application of CXCL1 followed by application of CXCL1, CXCL2, or LTB4).

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