Science Immunology

Supplementary Materials

The PDF file includes:

  • Materials and Methods
  • Fig. S1. Surface, cycling, and total CTLA-4 expression by Treg and Tconv.
  • Fig. S2. Effects of CTLA-4 blockade and inhibition of lysosomal acidification on TE.
  • Fig. S3. Internalization of CD80-GFP by CTLA-4–sufficient Treg.
  • Fig. S4. TE by resting and effector Treg in response to different concentrations of anti-CD3 Ab.
  • Fig. S5. CTLA-4 expression and cycling by resting and effector Treg.
  • Fig. S6. CTLA-4–mediated down-regulation of CD80-GFP on BM-derived DCs.
  • Fig. S7. GFP acquisition correlates with ICOS and CTLA-4 expression.
  • Fig. S8. CTLA-4 expression is increased at sites of self-antigen recognition, and ICOS marks Treg with highest CTLA-4.
  • Fig. S9. CTLA-4 targets expression of CD80 and CD86 on cDCs but not macrophages.
  • Fig. S10. Gating strategy for identification of splenic cDC subsets.
  • Fig. S11. Gating strategy for identification of LN resident and migratory cDC subsets.
  • Fig. S12. Phenotype of Tconv and Tregs in CTLA-4–deficient mice.
  • Fig. S13. CD80 and CD86 expression on splenic cDC subsets after CTLA-4 ablation.
  • Fig. S14. Functional identification of migratory DCs by FITC skin painting.
  • Fig. S15. Comparison of CD80 and CD86 expression in settings of CTLA-4 blockade and RAG deficiency.
  • Fig. S16. CD80 and CD86 expression on splenic cDC subsets in Rag2-deficient mice with or without T cell transfer.
  • Fig. S17. Impact of Treg expansion on CD80 and CD86 expression on cDC subsets.
  • Reference (63)

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Other Supplementary Material for this manuscript includes the following:

  • Table S1 (Microsoft Excel format). Raw data file.

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