Science Immunology

Supplementary Materials

The PDF file includes:

  • Fig. S1. Gating strategy for identification of ILC2 populations, related to Fig. 1 (A to C).
  • Fig. S2. Quantification of ILC2s in different tissues, related to Fig. 1 (A to C).
  • Fig. S3. Gating strategy for identification of ILC2 populations based on GATA-3 expression, related to Fig. 1 (A to C).
  • Fig. S4. Distribution of ILC2s and CD4+ T cells in rIL-33–treated mice lungs, related to Fig. 1 (D and F).
  • Fig. S5. Chemokine receptor expression on ILCs, related to Fig. 4 (A and B).
  • Fig. S6. Phenotype and IL-13 production by human ILC2 lines, related to Figs. 4 and 5.
  • Fig. S7. ILC2 motility on ECM proteins, collagen-III, and proteoglycans, versican and tenascin-C, related to Fig. 5 (A to C).
  • Fig. S8. BAPN fails to affect ILC2 cytokine production and eosinophil movement, related to Fig. 8G.
  • Table S1. Antibodies used for imaging of PCLS.
  • Table S2. Antibodies used for flow cytometry.
  • Table S3. Antibodies used for sorting human ILC2.

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Other Supplementary Material for this manuscript includes the following:

  • Movie S1 (.mp4 format). Time-lapse imaging of rIL-33–treated mouse lung PCLSs showing ILC2 movement and location, related to Fig. 2A.
  • Movie S2 (.mp4 format). Time-lapse imaging of rIL-33–treated mouse lung PCLSs depicting differences in movement of independent ILC2s, related to Fig. 2B.
  • Movie S3 (.mp4 format). Time-lapse imaging of rIL-33–treated mouse lung PCLSs depicting high-power videos differences in movement of independent ILC2s, related to Fig. 2C.
  • Movie S4 (.mp4 format). Intravital imaging of rIL-33–treated mouse lung depicting amoeboid ILC2 movement, related to Fig. 2H.
  • Movie S5 (.mp4 format). Influence of ILC2 survival and cytokines on ILC2 motility, related to Fig. 5 (A to C).
  • Movie S6 (.mp4 format). Time-lapse imaging of lung PCLSs from rIL-33–treated mouse treated with anti-CCR8 Ab or isotype Ab, related to Fig. 4I.
  • Movie S7 (.mp4 format). Collagen-I–induced ILC2 shape change and elongation of cell body, related to Fig. 5A.
  • Movie S8 (.mp4 format). Time-lapse imaging of a lung PCLSs assessing the effect of BAPN on rIL-33–treated mouse lung eosinophil movement, related to Fig. 8G.

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