Science Immunology

Supplementary Materials

The PDF file includes:

  • Materials and Methods
  • Fig. S1. In silico identification of candidate markers for BM and PB NCMs.
  • Fig. S2. Anti–PD-L1 antibody is preferable to Cx3cr1gfp/+ mice to mark circulating NCMs.
  • Fig. S3. Anti-CD43 antibody does not specifically mark circulating NCMs.
  • Fig. S4. PD-L1 expression is strongly reduced in Nr4a1−/− mice lacking NCMs.
  • Fig. S5. Kinetics and concentration of the anti–PD-L1 antibody for in vivo labeling of NCMs.
  • Fig. S6. CD43 does not specifically distinguish NCMs in the BM.
  • Fig. S7. CMs are the main monocyte subset in physical contact with TZ vessels in the diaphysis, whereas NCMs are in proximity of sinusoids.
  • Fig. S8. Transfer of purified CMs and BrdU pulse-chase experiment.
  • Fig. S9. PD-L1 expression is maintained on NCMs under inflammatory conditions, and PD-L1+ NCMs are found in TLOs.
  • Fig. S10. Anti–PD-L1 treatment does not deplete circulating NCMs.
  • Table S1. Association between the number of PD-L1+ cells and vessel subtypes.
  • Table S2. List of antibodies.

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Other Supplementary Material for this manuscript includes the following:

  • Movie S1 (.mov format). In vivo tracking of patrolling PD-L1+ NCMs in the cremaster microcirculation.
  • Data file S1 (Microsoft Excel format). List of genes differentially expressed in CMs and NCMs.
  • Data file S2 (Microsoft Excel format). Raw data from main figures.
  • Data file S3 (Microsoft Excel format). Raw data from supplementary figures.

Files in this Data Supplement: