Science Immunology

Supplementary Materials

The PDF file includes:

  • Fig. S1. Representative flow cytometry gating strategies used to identify T cell subsets in human blood and skin.
  • Fig. S2. CD103 expression is not induced on human CD4+ T cells in NSG mice.
  • Fig. S3. CyTOF analysis of CLA+ T cells in PBMCs.
  • Fig. S4. Frequencies of CLA+ T cell subsets in blood and skin.
  • Fig. S5. Experimental schematic of cell isolation and sort gates for RNA-seq.
  • Fig. S6. Gene expression in the different populations of CD4+CLA+ T cells from blood and skin as determined by RNA-seq.
  • Fig. S7. CLA+CD103+ T cells from human blood and skin coproduce IL-22 and IL-13.
  • Fig. S8. Analysis of TCRβ repertoire overlap and Vβ gene usage in CLA+ T cells from blood and skin.
  • Fig. S9. Human skin–derived immune cells do not infiltrate murine skin.

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Other Supplementary Material for this manuscript includes the following:

  • Table S1 (Microsoft Excel format). Detailed list of antibodies and reagents.
  • Table S2 (Microsoft Excel format). RNA-seq pairwise comparisons.
  • Table S3 (Microsoft Excel format). Raw data file.

Files in this Data Supplement: