Science Immunology

Supplementary Materials

The PDF file includes:

  • Fig. S1. MD simulation of DS-A2.
  • Fig. S2. Dipeptide-mediated folding efficiency of DS-MHC molecules.
  • Fig. S3. Peptide binding measurements and comparative purification profiles.
  • Fig. S4. Gating strategies for flow cytometry analysis.
  • Fig. S5. Purity and biotinylation efficiency.
  • Fig. S6. Detection of virus-derived antigen-specific T cells from donor PBMCs in a combinatorial encoding strategy.
  • Fig. S7. Detection of low-avidity pMHC-TCR interactions using DS–H-2Kb multimers.
  • Fig. S8. T cell detection plots for the stability analysis of DS-A2 empty-loadable tetramers.
  • Fig. S9. T cell detection plots of peptide dissociation analysis.
  • Table S1. Crystal structure data collection and refinement statistics.
  • Table S2. Protein yield after in vitro folding and purification of wt and DS-A2 molecules.

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