Science Immunology

Supplementary Materials

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  • Methods
  • Fig. S1. Binding of multiple different soluble TCRs and bsTCR bs-868Z11-CD3 to nonloaded DS-A*02:01 or DS-A*02:01 loaded with an irrelevant peptide.
  • Fig. S2. Analysis of DS-A*02:01 peptide receptiveness after different storage durations at −80°C measured by fluorescence anisotropy.
  • Fig. S3. Illustration of bsTCR bs-868Z11-CD3 construct.
  • Fig. S4. Rmax values reported for immobilized DS-A*02:01 and UV exchange generated WT-A*02:01 pMHCs.
  • Fig. S5. Analysis of UV exchange efficiency and Octet measurement results for 28 different peptides selected from SLYNTVATL-based positional scanning library.
  • Fig. S6. Octet binding kinetics measurements for DS-A*02:01 SLYNTVATL pMHC with immobilized bs-868Z11-CD3 directly after exchange and after 2 weeks of storage at 4°C.
  • Fig. S7. Flow cytometric peptide binding assay with an anti-human HLA-A2 antibody staining of T2 cells after exogenous peptide loading.
  • Table S1. Data collection and refinement statistics 1G4/DS-A*02:01/ESO 9V.
  • Table S2. bs-868Z11-CD3 binding affinity against SV9 peptide SLYNTVATL and peptides from positional scanning library.
  • Table S3. Cross-reactive peptide ligand search motif for bs-868Z11-CD3 based on the binding affinities measured using the positional scanning library.
  • Table S4. bs-868Z11-CD3 binding affinity for selected peptide ligands identified on the basis of the bs-868Z11-CD3 binding motif.

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